Functional variations of organic cation transporters associated to inflammatory bowel disease

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dc.contributor.authorSerrano León, Alejandra
dc.contributor.examiningcommitteeAukema, Harold (Human Nutritional Sciences) Bezabeh, Tedros (Human Nutritional Sciences)en_US
dc.contributor.supervisorEck, Peter (Human Nutritional Sciences)en_US
dc.date.accessioned2013-09-11T15:29:27Z
dc.date.available2013-09-11T15:29:27Z
dc.date.issued2013-09-11
dc.degree.disciplineHuman Nutritional Sciencesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractPolymorphisms in organic cation transporters SLC22A4, SLC22A23 and IBD5 locus have been associated with pathogenesis of inflammatory bowel disease (IBD). We sought to investigate the association of polymorphisms in these genes to IBD risk in a Canadian population, subclone and express human SLC22A23 gene to determine the localization in the cell. DNA samples from 160 patients with Crohn´s disease (CD), 149 patients with ulcerative colitis (UC) and 142 healthy controls were genotyped by PCR-RFLP analysis or TaqMan system. Gateway® recombination technology was used to transform and express SLC22A23 gene in HEK 293 cell line. Polymorphisms in the IBD5 locus rs17622208-AA genotype and rs11739135-CC genotype increase the risk of CD. Moreover, carriers of SLC22A23 polymorphisms rs4959235-TT genotype and rs9503518-GG genotype increase dramatically the risk of UC. We confirm that SLC22A23 polymorphisms are important in the pathogenesis of IBD and they can ultimately be used as biomarkers of the disease risk.en_US
dc.description.noteOctober 2013en_US
dc.identifier.urihttp://hdl.handle.net/1993/22167
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectinflammatory bowel diseaseen_US
dc.subjectorganic cation transportersen_US
dc.subjectSLC22A23en_US
dc.subjectpolymorphismsen_US
dc.titleFunctional variations of organic cation transporters associated to inflammatory bowel diseaseen_US
dc.typemaster thesisen_US
local.subject.manitobayesen_US
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