Comparative redox proteomics to investigate role of Nox mediated redox signaling in Fusarium graminearum pathogenesis
| dc.contributor.author | Joshi, Manisha | |
| dc.contributor.examiningcommittee | Wilkins, John (Medicine and Immunology) Gietz, Daniel (Biochemistry and Medical Genetics) | en_US |
| dc.contributor.supervisor | Rampitsch, Christof (Biochemistry and Medical Genetics) | en_US |
| dc.date.accessioned | 2011-08-09T18:09:35Z | |
| dc.date.available | 2011-08-09T18:09:35Z | |
| dc.date.issued | 2011-08-09 | |
| dc.degree.discipline | Biochemistry and Medical Genetics | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Fusarium graminearum causes Fusarium Head Blight, (one of) the most destructive cereal diseases in Canada. Yield loss, quality degradation and mycotoxin production make Fusarium a multifaceted threat. Regulated production of reactive oxygen species by Nox enzymes is indispensable for fungal pathogenesis. F. graminearum Nox mutant ∆noxAB produced equivalent mycotoxin but caused reduced virulence than wild-type. We hypothesized that Nox mediated redox signaling may participate in F. graminearum pathogenicity. Two-DE and gel-free biotin affinity chromatography, followed by LC-MS/MS analysis were employed for a comparative redox-proteomics analysis between wild-type and ∆noxAB to identify proteins oxidized by Nox activity. Total 35 proteins, 10 by 2-DE and 29 by gel-free system, were identified. 34% proteins participated in fungal metabolism, 20% in electron transfer reactions and 9% were anti-oxidant proteins. The findings suggested that Nox mediated thiol-disulfide exchange in proteins provide a switch for redox-dependent regulation of metabolic and developmental processes during induction of FHB. | en_US |
| dc.description.note | October 2011 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/4750 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | Fusarium graminearum | en_US |
| dc.subject | Fusarium Head Blight | en_US |
| dc.subject | Proteomics | en_US |
| dc.subject | NADPH oxidases | en_US |
| dc.subject | Redox signaling | en_US |
| dc.subject | Cysteine | en_US |
| dc.subject | mBBr | en_US |
| dc.subject | Pathogenesis | en_US |
| dc.title | Comparative redox proteomics to investigate role of Nox mediated redox signaling in Fusarium graminearum pathogenesis | en_US |
| dc.type | master thesis | en_US |