Development of sustainable microbial fermentation strategies for the production of medium-chain length polyhydroxyalkanoates (mcl-PHAs) from biodiesel derived glycerol
| dc.contributor.author | Sharma, Umesh | |
| dc.contributor.examiningcommittee | Levin, David (Biosystems Engineering) Sparling, David (Microbiology) | en_US |
| dc.contributor.supervisor | Cicek, Nazim (Biosystems Engineering) | en_US |
| dc.date.accessioned | 2015-08-06T19:21:10Z | |
| dc.date.available | 2015-08-06T19:21:10Z | |
| dc.date.issued | 2015-08-06 | en_US |
| dc.degree.discipline | Biosystems Engineering | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Bio-plastics have emerged as a promising alternative to conventional petrochemical derived plastics particularly over the past few decades. Numerous production methods for bio-plastics have been researched; however, work remains towards developing a commercially viable and economical process. The purpose of this research was to develop a sustainable fermentation strategy for production and scale-up of medium-chain length polyhydroxyalkanoates (mcl-PHAs), or bio-plastics, using a novel strain of the gram negative bacterium Pseudomonas putida, LS46, with biodiesel derived waste glycerol (WG) as feedstock. Experiments were conducted to gain a basic understanding of the general growth patterns exhibited by LS46. Thereafter, flask-batch experiments were conducted to study effects of variation in media conditions upon cell biomass production and mcl-PHA accumulation. Subsequently, optimal medium conditions observed within flasks were scaled-up and employed in the operation of a pilot-scale fermenter to increase production capacity for mcl-PHAs. It was concluded that mcl-PHA production at commercial levels could be viable with advanced process optimization. | en_US |
| dc.description.note | October 2015 | en_US |
| dc.identifier.citation | Not applicable | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/30658 | |
| dc.language.iso | eng | en_US |
| dc.publisher | Not applicable | en_US |
| dc.rights | open access | en_US |
| dc.subject | PHAs | en_US |
| dc.subject | Polyhydroxyalkanoates | en_US |
| dc.subject | Glycerol | en_US |
| dc.subject | Bioplastics | en_US |
| dc.title | Development of sustainable microbial fermentation strategies for the production of medium-chain length polyhydroxyalkanoates (mcl-PHAs) from biodiesel derived glycerol | en_US |
| dc.type | master thesis | en_US |
| local.subject.manitoba | yes | en_US |