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dc.contributor.supervisorde Kievit, Teresa (Microbiology) Loewen, Peter (Microbiology)en_US
dc.contributor.authorChan, Jason Hok Shun
dc.date.accessioned2012-09-20T16:45:30Z
dc.date.available2012-09-20T16:45:30Z
dc.date.issued2012-09-20
dc.identifier.urihttp://hdl.handle.net/1993/8895
dc.description.abstractPseudomonas chlororaphis PA23 is a promising biological control candidate against Sclerotinia sclerotiorum, a fungal pathogen that causes stem rot in canola. A library of transposon mutants was previously created to understand the molecular mechanisms underlying the antifungal capabilities of PA23. A novel LysR-type transcriptional regulator, called PtrA, was identified as a key global regulator involved in secondary metabolite production. The function of PtrA at the molecular level was investigated in this thesis. Solubility problems encountered during the purification of PtrA redirected efforts to studying a truncated version of the protein instead. A two-step purification of the truncated protein, involving streptomycin sulfate precipitation and immobilized metal-ion affinity chromatography, yielded a highly pure protein. Preliminary crystal growth was achieved for the effector binding domain portion of PtrA. Transcriptional fusions suggested that essential regulatory binding sites of ptrA may lie somewhere between 52 and 198 bp upstream of the translational start site. The research presented in this thesis will help guide future functional studies on PtrA.en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectptrAen_US
dc.subjectLTTRen_US
dc.subjectPseudomonas chlororaphisen_US
dc.titleFunctional investigation of a transcriptional regulator ptrA from Pseudomonas chlororaphis PA23en_US
dc.typeinfo:eu-repo/semantics/masterThesis
dc.typemaster thesisen_US
dc.degree.disciplineMicrobiologyen_US
dc.contributor.examiningcommitteeMark, Brian (Microbiology) McKenna, Sean (Chemistry)en_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.noteOctober 2012en_US


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