Home

Stachybotrys chartarum spore extracts and their effects on surfactant protein expression and surfactant quantity and quality in fetal rat lung epithelial cells

Show simple item record

dc.contributor.supervisor Thliveris, J.A. (Human Anatomy and Cell Science) Scott, J.E. (Oral Biology) en_US
dc.contributor.author Pollard, Gail F.
dc.date.accessioned 2012-08-29T14:58:26Z
dc.date.available 2012-08-29T14:58:26Z
dc.date.issued 2012-08-29
dc.identifier.uri http://hdl.handle.net/1993/8584
dc.description.abstract S. chartarum spores are prevalent in buildings affected by flooding and have been tied to occurrences of pulmonary hemosiderosis in infants in Cleveland, Ohio, several years ago. Rationale: Past studies have focused on the effects of S. chartarum spores on surfactant in terms of composition and quantity and very little has been done to investigate the effects of S. chartarum extracts on surfactant protein expression in the fetal lung. Methods: Spore extracts were incubated with cultures of human lung A549 cells, fetal rat lung type II and fibroblast cells. MTT formazan assays analyzed cell viability. The expression of surfactant protein A and surfactant protein B was assessed using western blotting results. Captive bubble surfactometry investigated the quality of surfactant in terms of surface tension and bubble volume. Electron microscopy examined changes in cellular structure of treated cells. Conclusions: Exposure to S. chartarum extracts had negative effects on cell viability, expression of surfactant proteins A and B and the production and quality of pulmonary surfactant. Spore associated changes were apparent in the lamellar bodies of fetal rat lung alveolar type II, and human A549 cells. en_US
dc.rights info:eu-repo/semantics/openAccess
dc.subject Stachybotrys chartarum en_US
dc.subject captive bubble en_US
dc.title Stachybotrys chartarum spore extracts and their effects on surfactant protein expression and surfactant quantity and quality in fetal rat lung epithelial cells en_US
dc.type info:eu-repo/semantics/masterThesis
dc.degree.discipline Human Anatomy and Cell Science en_US
dc.contributor.examiningcommittee Shaw, Anthony (National Research Council) Choo-Smith, Lin-Ping (National Research Council) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note October 2012 en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

View Statistics