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dc.contributor.author Sandhu, Sumit
dc.contributor.author Wu, Xiaoli
dc.contributor.author Nabi, Zinnatun
dc.contributor.author Rastegar, Mojgan
dc.contributor.author Kung, Sam
dc.contributor.author Mai, Sabine
dc.contributor.author Ding, Hao
dc.date.accessioned 2012-06-01T16:51:59Z
dc.date.available 2012-06-01T16:51:59Z
dc.date.issued 2012-03-27
dc.identifier.citation Molecular Cancer. 2012 Mar 27;11(1):18
dc.identifier.uri http://hdl.handle.net/1993/7249
dc.description.abstract Abstract Background HLTF (Helicase-like Transcription Factor) is a DNA helicase protein homologous to the SWI/SNF family involved in the maintenance of genomic stability and the regulation of gene expression. HLTF has also been found to be frequently inactivated by promoter hypermethylation in human colon cancers. Whether this epigenetic event is required for intestinal carcinogenesis is unknown. Results To address the role of loss of HLTF function in the development of intestinal cancer, we generated Hltf deficient mice. These mutant mice showed normal development, and did not develop intestinal tumors, indicating that loss of Hltf function by itself is insufficient to induce the formation of intestinal cancer. On the Apcmin/+ mutant background, Hltf- deficiency was found to significantly increase the formation of intestinal adenocarcinoma and colon cancers. Cytogenetic analysis of colon tumor cells from Hltf -/-/Apcmin/+ mice revealed a high incidence of gross chromosomal instabilities, including Robertsonian fusions, chromosomal fragments and aneuploidy. None of these genetic alterations were observed in the colon tumor cells derived from Apcmin/+ mice. Increased tumor growth and genomic instability was also demonstrated in HCT116 human colon cancer cells in which HLTF expression was significantly decreased. Conclusion Taken together, our results demonstrate that loss of HLTF function promotes the malignant transformation of intestinal or colonic adenomas to carcinomas by inducing genomic instability. Our findings highly suggest that epigenetic inactivation of HLTF, as found in most human colon cancers, could play an important role in the progression of colon tumors to malignant cancer.
dc.title Loss of HLTF function promotes intestinal carcinogenesis
dc.type Journal Article
dc.language.rfc3066 en
dc.description.version Peer Reviewed
dc.rights.holder Sumit Sandhu et al.; licensee BioMed Central Ltd.
dc.date.updated 2012-06-01T16:51:59Z
dc.identifier.doi http://dx.doi.org/10.1186/1476-4598-11-18


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