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dc.contributor.supervisor Burczynski, Frank J (Pharmacy) en_US
dc.contributor.author Chen, Yufei
dc.date.accessioned 2012-04-05T15:22:42Z
dc.date.available 2012-04-05T15:22:42Z
dc.date.issued 2012-04-05
dc.identifier.uri http://hdl.handle.net/1993/5260
dc.description.abstract Liver fatty acid binding protein has been reported to possess antioxidant properties in the liver. The aim of this study was to investigate the effect of this protein in a nonalcoholic fatty liver disease (NAFLD) cell culture model. Rat hepatoma cells were treated with an oleate:palmitate (2:1) mixture for either 1 and 2 days, or further treated with 500 µM clofibrate to induce L-FABP expression. Intracellular lipid accumulation was quantitated by Nile Red. Lipotoxicity was determined using the WST-1 assay. Dichlorofluorescein (DCF) was utilized to assess intracellular reactive oxidative species (ROS) level. Measurement of lipotoxicity showed statistical decreases in cell viability as lipid concentrations increased in a dose-dependent manner. NAFLD cell cultures showed characteristic cellular damage from increased ROS levels in fatty acid treated cells. All groups treated with clofibrate showed statistically increased intracellular L-FABP levels and reduced ROS levels. The results lead to the conclusion that clofibrate induces L-FABP expression and in this manner suppresses hepatocellular ROS generation. en_US
dc.rights info:eu-repo/semantics/openAccess
dc.subject L-FABP en_US
dc.subject NAFLD en_US
dc.subject ROS en_US
dc.subject antioxidant therapy en_US
dc.subject oxidative stress en_US
dc.title Role of liver fatty acid binding protein in fatty liver cell culture model en_US
dc.type info:eu-repo/semantics/masterThesis
dc.degree.discipline Pharmacy en_US
dc.contributor.examiningcommittee Gong, Yuewen (Pharmacy) Minuk, Gerald Y (Pharmacology and Therapeutics) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note May 2012 en_US


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