Show simple item record

dc.contributor.supervisorBurczynski, Frank J (Pharmacy)en_US
dc.contributor.authorChen, Yufei
dc.date.accessioned2012-04-05T15:22:42Z
dc.date.available2012-04-05T15:22:42Z
dc.date.issued2012-04-05
dc.identifier.urihttp://hdl.handle.net/1993/5260
dc.description.abstractLiver fatty acid binding protein has been reported to possess antioxidant properties in the liver. The aim of this study was to investigate the effect of this protein in a nonalcoholic fatty liver disease (NAFLD) cell culture model. Rat hepatoma cells were treated with an oleate:palmitate (2:1) mixture for either 1 and 2 days, or further treated with 500 µM clofibrate to induce L-FABP expression. Intracellular lipid accumulation was quantitated by Nile Red. Lipotoxicity was determined using the WST-1 assay. Dichlorofluorescein (DCF) was utilized to assess intracellular reactive oxidative species (ROS) level. Measurement of lipotoxicity showed statistical decreases in cell viability as lipid concentrations increased in a dose-dependent manner. NAFLD cell cultures showed characteristic cellular damage from increased ROS levels in fatty acid treated cells. All groups treated with clofibrate showed statistically increased intracellular L-FABP levels and reduced ROS levels. The results lead to the conclusion that clofibrate induces L-FABP expression and in this manner suppresses hepatocellular ROS generation.en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectL-FABPen_US
dc.subjectNAFLDen_US
dc.subjectROSen_US
dc.subjectantioxidant therapyen_US
dc.subjectoxidative stressen_US
dc.titleRole of liver fatty acid binding protein in fatty liver cell culture modelen_US
dc.typeinfo:eu-repo/semantics/masterThesis
dc.typemaster thesisen_US
dc.degree.disciplinePharmacyen_US
dc.contributor.examiningcommitteeGong, Yuewen (Pharmacy) Minuk, Gerald Y (Pharmacology and Therapeutics)en_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.noteMay 2012en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record