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dc.contributor.supervisor Hombach-Klonisch, Sabine (Human Anatomy and Cell Science) en_US
dc.contributor.author Danescu, Adrian
dc.date.accessioned 2012-04-04T14:20:20Z
dc.date.available 2012-04-04T14:20:20Z
dc.date.issued 2012-04-04
dc.identifier.uri http://hdl.handle.net/1993/5246
dc.description.abstract Early diagnosis of endometrial cancer (EC) is uncertain and women undergo preventive hysterectomy in cases where a non-invasive treatment can be used instead. To contribute to solving this challenge we investigated if early changes in the nuclear 3D telomere architecture during carcinoma development can be detected prior to the first morphological evidence of precancerous lesions. We utilized Pten heterozygous mice that develop progressive carcinoma in the endometrial tissue similar to EC development in women. We used telomere fluorescence in situ hybridization (FISH), 3D molecular imaging and analysis techniques on interphase nuclei of endometrial glandular epithelial cells to identify alterations in the 3D-telomere profile. We found that telomere dysfunction in Pten heterozygous mice is present already in endometrial simple hyperplasia lesions prior to detectable loss of PTEN protein expression and that the 3D telomere architecture has a specific signature that indicates early telomere dysfunction predictive for endometrial malignant transformation. en_US
dc.rights info:eu-repo/semantics/openAccess
dc.subject endometrial cancer en_US
dc.subject 3D telomere profile en_US
dc.subject telomere fluorescence in-situ hybridization (telomere-FISH) en_US
dc.subject PTEN en_US
dc.subject nuclear architecture en_US
dc.title The 3D nuclear organization of telomeres during endometrial carcinoma development en_US
dc.type info:eu-repo/semantics/masterThesis
dc.degree.discipline Human Anatomy and Cell Science en_US
dc.contributor.examiningcommittee Simard, Louise (Biochemistry and Medical Genetics) Mai, Sabine (Biochemistry & Medical Genetics) Scott, J. Elliott (Oral Biology) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note May 2012 en_US


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