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dc.contributor.supervisor Freed, Darren (Physiology) Arora, Rakesh (Surgery) en_US
dc.contributor.author Ngo, Melanie Allison
dc.date.accessioned 2012-01-10T17:15:00Z
dc.date.available 2012-01-10T17:15:00Z
dc.date.issued 2012-01-10
dc.identifier.uri http://hdl.handle.net/1993/5059
dc.description.abstract There is emerging evidence to suggest that cardiac myofibroblasts (CMyfbs) participating in cardiac fibrosis represent a heterogeneous population in origin. We hypothesized that bone marrow derived mesenchymal stem cells (MSCs) readily adopt a myofibroblastic phenotype in culture. We assessed and compared human primary MSCs and human CMyfbs with respect to their phenotypic and functional characteristics by examining their gene expression profile, ability to contract collagen gels, and ability to synthesize collagen. We also examined the role of non-muscle myosin II (NMMII) in modulating the myofibroblast function using siRNA and blebbistatin to inhibit NMMII activity. The data revealed that MSCs adopt a myofibroblastic phenotype in culture and demonstrate the capability to contract collagen gels and synthesize collagen similar to human CMyfbs. Inhibition of NMMII activity with blebbistatin completely inhibits gel contractility without affecting cell viability. Thus, MSCs exhibit similar physiological and functional characteristics as CMyfbs, and may contribute to cardiac fibrosis. en_US
dc.subject mesenchymal stem cells en_US
dc.subject myofibroblasts en_US
dc.title Human mesenchymal stem cells express a myofibroblastic phenotype in vitro en_US
dc.degree.discipline Physiology en_US
dc.contributor.examiningcommittee Dixon, Ian (Physiology) Wigle, Jeffrey (Biochemistry & Medical Genetics) Fernyhough, Paul (Pharmacology) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note February 2012 en_US


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