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Molecular characterization of ciprofloxacin resistant and/or beta-lactamase producing Escherichia coli from the Vancouver Coastal Health region

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dc.contributor.supervisor Mulvey, Michael (Medical Microbiology) en_US
dc.contributor.author Gonsalves, Elizabeth A.
dc.date.accessioned 2011-09-12T14:34:59Z
dc.date.available 2011-09-12T14:34:59Z
dc.date.issued 2011-09-12
dc.identifier.uri http://hdl.handle.net/1993/4886
dc.description.abstract Emergent multidrug resistant Escherichia coli increase clinical challenges. This thesis describes the resistance patterns, molecular epidemiology and mechanisms, for 315 E. coli from patients in the Vancouver Coastal Health Region for 2006/2007. Automated susceptibility testing was confirmed via E-test® for AmpC and/or ESBL production. PFGE, RFLP and PCR were used to assess genotypic relationships, and plasmid character. AmpC production was facilitated mainly by promoter mutations (54.5%). The principal ESBL detected was CTX-M-15 (49.5%). An unidentified ESBL-producer, with a pI near 8.3, was detected. A plasmid displayed variant resistance phenotypes dependent on selective growth media. A positive correlation between ST131 with CTX-M-15 and CIPR indicated the dissemination of companion phenotypes. Ciprofloxacin resistance resulted mainly (98.0%) from a double gyrA mutation. Overall fluoroquinolone resistance was not assessable due to exclusive selection parameters in this evaluation. Fluoroquinolone resistance factors require further examination to understand what causes resistant phenotypes exclusive of chromosomal mutations. ii en_US
dc.subject epidemiology en_US
dc.subject resistance en_US
dc.subject fluoroquinolone en_US
dc.subject ciprofloxacin en_US
dc.subject beta-lactamase en_US
dc.subject ESBL en_US
dc.subject E. coli en_US
dc.subject AmpC en_US
dc.title Molecular characterization of ciprofloxacin resistant and/or beta-lactamase producing Escherichia coli from the Vancouver Coastal Health region en_US
dc.degree.discipline Medical Microbiology en_US
dc.contributor.examiningcommittee Graham, Morag (Medical Microbiology) Wylie John (Medical Microbiology) Worobec, Elizabeth (Microbiology) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note October 2011 en_US


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