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dc.contributor.supervisor Holley, Richard (Food Science) en_US
dc.contributor.author Nilson, Anna
dc.date.accessioned 2011-08-30T16:14:14Z
dc.date.available 2011-08-30T16:14:14Z
dc.date.issued 2011-08-30
dc.identifier.uri http://hdl.handle.net/1993/4790
dc.description.abstract Escherichia (E.) coli O157:H7 survival in dry cured (uncooked) meat products leading to human illness outbreaks is an international problem. Their manufacture does not involve a heat kill step to ensure the destruction of the organism, and the adverse conditions created during processing may not be sufficient to prevent E. coli O157:H7 survival. Deodorized yellow mustard powder has antimicrobial properties from glucosinolate (sinalbin) hydrolysis catalyzed by the endogenous enzyme myrosinase, generating antimicrobial isothiocyanate (PHBIT). Previous work has shown that its addition during dry sausage manufacture was capable of eliminating the pathogen. In this study, its use for the same purpose was investigated during dry cured Westphalian ham production. Hams were inoculated with a 7.5 log cfu•g-1 cocktail of E. coli O157:H7, surface applied with 4% or 6% (w/w) deodorized yellow mustard powder, and monitored for E. coli O157:H7 survival during 80d ham maturation. One trial included the inoculation of Staphylococcus (S.) carnosus, a meat starter culture with myrosinase-like activity, onto the hams (after salt equilibration) to accelerate formation of antimicrobial isothiocyanate from mustard glucosinolate and help control the pathogen. In both trials, E. coli O157:H7 was reduced 3 log cfu•g-1 by 21d on hams treated with mustard powder, whereas only a 1 log cfu•g-1 reduction was found in the inoculated control which was not treated with mustard. By 45d, hams treated with mustard powder showed a reduction of >5 log cfu•g-1 E. coli O157:H7, whereas it took 80d to for numbers in control hams to be similarly reduced. Since a 5 log kill of E. coli O157:H7 was achieved in control hams by the end of 80d, dry cured Westphalian ham manufacture would be considered capable of controlling the risk of E. coli O157:H7 survival by North American regulatory agencies. However, deodorized yellow mustard powder at 4%, and to a greater extent at 6%, eliminated the pathogen at a significantly faster rate than the control during ham processing. Addition of the S. carnosus starter culture in trial 2 may have contributed to the maintenance of this effect through isothiocyanate formation. It also helped restore numbers of staphylococci, which were found to be sensitive to deodorized mustard powder. en_US
dc.subject Deodorized yellow mustard powder en_US
dc.subject E. coli O157:H7 en_US
dc.subject Dry cured ham en_US
dc.subject p-hydroxybenzyl isothiocyanate en_US
dc.title Use of deodorized yellow mustard powder to control Escherichia coli O157:H7 in dry cured Westphalian ham en_US
dc.degree.discipline Food Science en_US
dc.contributor.examiningcommittee Blank, Greg (Food Science) Sparling, Richard (Microbiology) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note October 2011 en_US


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