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dc.contributor.supervisor Rampitsch, Christof (Biochemistry and Medical Genetics) en_US
dc.contributor.author Joshi, Manisha
dc.date.accessioned 2011-08-09T18:09:35Z
dc.date.available 2011-08-09T18:09:35Z
dc.date.issued 2011-08-09
dc.identifier.uri http://hdl.handle.net/1993/4750
dc.description.abstract Fusarium graminearum causes Fusarium Head Blight, (one of) the most destructive cereal diseases in Canada. Yield loss, quality degradation and mycotoxin production make Fusarium a multifaceted threat. Regulated production of reactive oxygen species by Nox enzymes is indispensable for fungal pathogenesis. F. graminearum Nox mutant ∆noxAB produced equivalent mycotoxin but caused reduced virulence than wild-type. We hypothesized that Nox mediated redox signaling may participate in F. graminearum pathogenicity. Two-DE and gel-free biotin affinity chromatography, followed by LC-MS/MS analysis were employed for a comparative redox-proteomics analysis between wild-type and ∆noxAB to identify proteins oxidized by Nox activity. Total 35 proteins, 10 by 2-DE and 29 by gel-free system, were identified. 34% proteins participated in fungal metabolism, 20% in electron transfer reactions and 9% were anti-oxidant proteins. The findings suggested that Nox mediated thiol-disulfide exchange in proteins provide a switch for redox-dependent regulation of metabolic and developmental processes during induction of FHB. en_US
dc.subject Fusarium graminearum en_US
dc.subject Fusarium Head Blight en_US
dc.subject Proteomics en_US
dc.subject NADPH oxidases en_US
dc.subject Redox signaling en_US
dc.subject Cysteine en_US
dc.subject mBBr en_US
dc.subject Pathogenesis en_US
dc.title Comparative redox proteomics to investigate role of Nox mediated redox signaling in Fusarium graminearum pathogenesis en_US
dc.degree.discipline Biochemistry and Medical Genetics en_US
dc.contributor.examiningcommittee Wilkins, John (Medicine and Immunology) Gietz, Daniel (Biochemistry and Medical Genetics) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note October 2011 en_US


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