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dc.contributor.supervisor Coombs, Kevin (Medical Microbiology) en
dc.contributor.author Awadh, Abdullah
dc.date.accessioned 2011-05-03T13:49:01Z
dc.date.available 2011-05-03T13:49:01Z
dc.date.issued 2011-05-03T13:49:01Z
dc.identifier.uri http://hdl.handle.net/1993/4597
dc.description.abstract Although the demand for high scale production of mammalian orthoreoviruses (T3D in particular) is mounting to advance the research studies and clinical trials associated with their oncolytic capacity, very few reported studies have tried to understand the factors that affect the its production and subsequently optimize them to maximize the virus yield in cell culture systems. For this study, we manipulated several growth parameters (multiplicity of infection, cell density, cell feeding, media pH, and flask size) sequentially to select optimal conditions. Manipulation of cell density, whether cells were fed or not, and flask size all led to moderate changes in progeny virus titer. Altered media pH led to dramatic (more than 100 fold) virus replication changes. We conclude that cell physiological status has a key impact on infection progression and virus production. Optimal parameters consisted of infection at MOI of 0.1, cell density at infection of 95%, media re-feeding, media pH 7.0-8.5, and the smaller the flask size the better. en
dc.format.extent 4937914 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.subject Cell Culture en
dc.subject Virology en
dc.title Optimization of mammalian reovirus T3D growth in L929 cells en
dc.degree.discipline Medical Microbiology en
dc.contributor.examiningcommittee Wylie, John (Medical Microbiology) Butler, Michael (Microbiology) en
dc.degree.level Master of Science (M.Sc.) en
dc.description.note May 2011 en


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