Show simple item record

dc.contributor.supervisorPlummer, Francis A. (Medical Microbiology)en
dc.contributor.authorSarna, Caitlin S.
dc.date.accessioned2011-04-14T19:54:46Z
dc.date.available2011-04-14T19:54:46Z
dc.date.issued2011-04-14T19:54:46Z
dc.identifier.urihttp://hdl.handle.net/1993/4589
dc.description.abstractHeterosexual intercourse now accounts for the majority of HIV transmission within sub-Saharan Africa. The generation of microbicides and vaccines, therefore, requires a better understanding of the mucosal correlates of protection, including the role of HIV-specific IgA. It is now accepted that not all individuals are equally susceptible to HIV-1 infection, as exemplified by the HIV Exposed Seronegative (HESN) women of the Pumwani Cohort in Nairobi, Kenya. To assess whether mucosal IgA responses contribute to this protection, 3 novel IgA variable genes were cloned from HESN cervical B-cell cDNA. Nine monoclonal IgA Abs were produced, two of which were properly produced from cell culture. The HESN-derived A6/30L and A9/30L variants had a greater specificity for gp120IIIB than their A6/4L and A9/4L counterparts, while the A6 variant recognizes a distinct gp120 epitope compared to the broadly neutralizing antibody IgGb12. Further characterization of these IgA chains may suggest their suitability for use in microbicides or mucosal vaccines.en
dc.format.extent1237514 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectIgAen
dc.subjectHIV-1en
dc.subjectgp120en
dc.subjectMonoclonal antibodiesen
dc.subjectHESNen
dc.subjectMucosal immunologyen
dc.titleCloning and characterization of novel IgA antibody variable heavy and light chains from HIV-1 resistant sex workers from Nairobi, Kenyaen
dc.typeinfo:eu-repo/semantics/masterThesis
dc.typemaster thesisen_US
dc.degree.disciplineMedical Microbiologyen_US
dc.contributor.examiningcommitteeFowke, Keith R. (Medical Microbiology) Berry, Jody D. (Immunology)en
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.noteMay 2011en


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record