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Cloning and characterization of novel IgA antibody variable heavy and light chains from HIV-1 resistant sex workers from Nairobi, Kenya

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dc.contributor.supervisor Plummer, Francis A. (Medical Microbiology) en
dc.contributor.author Sarna, Caitlin S.
dc.date.accessioned 2011-04-14T19:54:46Z
dc.date.available 2011-04-14T19:54:46Z
dc.date.issued 2011-04-14T19:54:46Z
dc.identifier.uri http://hdl.handle.net/1993/4589
dc.description.abstract Heterosexual intercourse now accounts for the majority of HIV transmission within sub-Saharan Africa. The generation of microbicides and vaccines, therefore, requires a better understanding of the mucosal correlates of protection, including the role of HIV-specific IgA. It is now accepted that not all individuals are equally susceptible to HIV-1 infection, as exemplified by the HIV Exposed Seronegative (HESN) women of the Pumwani Cohort in Nairobi, Kenya. To assess whether mucosal IgA responses contribute to this protection, 3 novel IgA variable genes were cloned from HESN cervical B-cell cDNA. Nine monoclonal IgA Abs were produced, two of which were properly produced from cell culture. The HESN-derived A6/30L and A9/30L variants had a greater specificity for gp120IIIB than their A6/4L and A9/4L counterparts, while the A6 variant recognizes a distinct gp120 epitope compared to the broadly neutralizing antibody IgGb12. Further characterization of these IgA chains may suggest their suitability for use in microbicides or mucosal vaccines. en
dc.format.extent 1237514 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.subject IgA en
dc.subject HIV-1 en
dc.subject gp120 en
dc.subject Monoclonal antibodies en
dc.subject HESN en
dc.subject Mucosal immunology en
dc.title Cloning and characterization of novel IgA antibody variable heavy and light chains from HIV-1 resistant sex workers from Nairobi, Kenya en
dc.degree.discipline Medical Microbiology en
dc.contributor.examiningcommittee Fowke, Keith R. (Medical Microbiology) Berry, Jody D. (Immunology) en
dc.degree.level Master of Science (M.Sc.) en
dc.description.note May 2011 en


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