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The use of RNA interference as a tool to examine gene function, and its potential as a species-specific pesticide in the yellow fever mosquito, Aedes aegypti

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dc.contributor.supervisor Whyard, Steven (Biological Sciences) en
dc.contributor.author Singh, Aditi Diana
dc.date.accessioned 2011-04-06T20:34:38Z
dc.date.available 2011-04-06T20:34:38Z
dc.date.issued 2011-04-06T20:34:38Z
dc.identifier.uri http://hdl.handle.net/1993/4444
dc.description.abstract RNA interference (RNAi) is a gene silencing mechanism induced by double-stranded RNA (dsRNA). RNAi has been used extensively to create loss-of-function mutants in many species to identify the functions of genes, but it also has the potential to be used as a species-specific pesticide if the dsRNA can silence essential genes in pests. The mosquito Aedes aegypti is a vector of numerous viruses including Dengue and West Nile virus, and is frequently controlled by chemical insecticides. With growing concerns about the extensive use of broad-spectrum pesticides, new control methods are eagerly sought. In this study, I examined the efficacy of feeding pesticidal dsRNAs to mosquito larvae. A dose-dependent RNAi response and mortality was observed when larvae were fed dsRNA targeting several different genes. Unlike RNAi in the related dipteran Drosophila melanogaster, RNAi in A. aegypti also appeared to be systemic, spreading beyond the gut to other tissues. A degree of species-specificity was also observed, as dsRNA specific to the D. melanogaster β-tubulin gene killed D. melanogaster larvae but did not kill mosquito larvae. RNAi was also used to determine the function of a newly-identified A. aegypti cytochrome P450 (CYP) gene, Aacyp. This gene showed male-biased expression in the mosquitoes, and was expressed primarily in the male abdomen and/or thorax, but unlike some other insect male-biased CYPs, Aacyp was not highly expressed in the reproductive structures. While dsRNA injections successfully knocked down expression of Aacyp, no discernable change in reproductive or male-specific behaviours were noted. Nevertheless, RNAi is still considered a highly versatile tool for both gene function studies and has promising potential to be developed into a novel class of pesticides. en
dc.format.extent 885582 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.subject RNA interference en
dc.subject mosquito control en
dc.title The use of RNA interference as a tool to examine gene function, and its potential as a species-specific pesticide in the yellow fever mosquito, Aedes aegypti en
dc.degree.discipline Biological Sciences en
dc.contributor.examiningcommittee Civetta, Alberto (Biochemistry & Medical Genetics) Schroeder, Dana (Biological Sciences) en
dc.degree.level Master of Science (M.Sc.) en
dc.description.note May 2011 en


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