Genetic analysis of resistance to Fusarium head blight in wheat (Triticum spp.) using phenotypic characters and molecular markers

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dc.contributor.supervisor Gilbert, Jeannie (Biological Sciences) en
dc.contributor.author Malihipour, Ali
dc.date.accessioned 2010-10-26T17:27:54Z
dc.date.available 2010-10-26T17:27:54Z
dc.date.issued 2010-10-26T17:27:54Z
dc.identifier.uri http://hdl.handle.net/1993/4284
dc.description.abstract Fusarium head blight (FHB), caused mainly by Fusarium graminearum (teleomorph: Gibberella zeae), is one of the most damaging diseases of wheat. A ‘Brio’/‘TC 67’ spring wheat population was used to map quantitative trait loci (QTLs) for resistance to FHB, and to study the association of morphological and developmental characteristics with FHB resistance. Interval mapping (IM) detected a major QTL on chromosome 5AL for resistance to disease severity (type II resistance) and Fusarium-damaged kernels (FDK) under greenhouse and field conditions, respectively. Inconsistent QTL(s) was also detected on chromosome 5BS for disease severity and index using field data. The associations of plant height and number of days to anthesis were negative with disease incidence, severity, index, and deoxynivalenol (DON) accumulation data under field conditions. However, number of days to anthesis was positively correlated with disease severity (greenhouse) and FDK (field). Awnedness had a negative effect on FHB, namely the presence of awns resulted in less disease in the population. Spike threshability also affected FHB so that the hard threshable genotypes represented lower disease. Phylogenetic relationships of putative F. graminearum isolates from different sources were characterized using Tri101 gene sequencing data. Canadian and Iranian isolates clustered in F. graminearum lineage 7 (=F. graminearum sensu stricto) within the F. graminearum clade while the isolates received from CIMMYT, Mexico were placed in F. graminearum lineage 3 (=Fusarium boothii) within the Fg clade or Fusarium cerealis. The PCR assay based on the Tri12 gene revealed the presence of the NIV, 3-ADON, and 15-ADON chemotypes with 15-ADON being the predominant chemotype. While we did not find the NIV chemotype among the Canadian isolates, it was the predominant chemotype among the Iranian isolates. High variation in aggressiveness was observed among and within Fusarium species tested, with the isolates of F. graminearum sensu stricto being the most aggressive and the NIV chemotype being the least aggressive. The interactions between Fusarium isolates and wheat genotypes from different sources were investigated by inoculating isolates of F. graminearum sensu stricto and F. boothii on wheat genotypes. Significant differences were observed among the genotypes inoculated by single isolates. Results also showed significant interactions between Fusarium isolates and wheat genotypes. The F. boothii isolates from CIMMYT produced low disease symptom and infection on wheat genotypes regardless of the origin of the genotypes while F. graminearum sensu stricto isolates from Canada and Iran resulted in higher FHB scores. en
dc.format.extent 7299263 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.subject Wheat en
dc.subject Triticum aestivum en
dc.subject Triticum timopheevii en
dc.subject Brio en
dc.subject TC 67 en
dc.subject Fusarium head blight en
dc.subject Quantitative trait loci en
dc.subject resistance en
dc.subject 5AL en
dc.subject 5BS en
dc.subject plant height en
dc.subject number of days to anthesis en
dc.subject awnedness en
dc.subject spike threshability en
dc.subject Tri101 en
dc.subject Fusarium graminearum en
dc.subject Fusarium boothii en
dc.subject Fusarium cerealis en
dc.subject Lineage 7 en
dc.subject Lineage 3 en
dc.subject Tri12 en
dc.subject NIV en
dc.subject 3-ADON en
dc.subject 15-ADON en
dc.subject aggressiveness en
dc.subject host-pathogen interaction en
dc.title Genetic analysis of resistance to Fusarium head blight in wheat (Triticum spp.) using phenotypic characters and molecular markers en
dc.degree.discipline Biological Sciences en
dc.contributor.examiningcommittee Brûlé-Babel, Anita (Plant Science) Piercey-Normore, Michele (Biological Sciences) Fedak, George (Biological Sciences) Xue, Steven (USDA-ARS, Fargo, ND) en
dc.degree.level Doctor of Philosophy (Ph.D.) en
dc.description.note February 2011 en

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