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dc.contributor.supervisorMowat, Michael (Biochemistry & Medical Genetics)en
dc.contributor.authorShields, Caroline
dc.date.accessioned2010-09-10T22:00:25Z
dc.date.available2010-09-10T22:00:25Z
dc.date.issued2010-09-10T22:00:25Z
dc.identifier.urihttp://hdl.handle.net/1993/4147
dc.description.abstractThe purpose of this project was to determine how Dlc-2 and Rho signaling modulate the ceramide induction of PGP synthase. This induction was studied at the transcriptional, post-transcriptional, and post-translational levels using cell culture, Real-Time RT-PCR, protein purification, phage display, and western blotting techniques. We have demonstrated that the PGP synthase gene is not controlled at the transcriptional level by ceramide and Rho, nor is the mRNA stability of PGP synthase affected. However, ceramide and Rho do seem to exhibit translational or post-translational control over the PGP synthase protein. The relationships between Dlc-2 (and Rho), ceramide, and PGP synthase (and CL) are important to understand. All three are involved in cancer and apoptotic responses. The knowledge gained by the experiments discussed in this thesis will contribute to an understanding of how these proteins and lipids interact. This knowledge may then be used in the future to develop cancer treatments.en
dc.format.extent5459123 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDlc-2en
dc.subjectPGP synthaseen
dc.subjectceramideen
dc.subjectcardiolipinen
dc.subjectRhoen
dc.subjectRhoGAPen
dc.titleThe role of Dlc-2 in ceramide signaling to PGP synthaseen
dc.typeinfo:eu-repo/semantics/masterThesis
dc.typemaster thesisen_US
dc.degree.disciplineBiochemistry and Medical Geneticsen_US
dc.contributor.examiningcommitteeDavie, Jim (Biochemistry & Medical Genetics) Hatch, Grant (Pharmacology & Therapeutics)en
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.noteOctober 2010en


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