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dc.contributor.supervisor Mowat, Michael (Biochemistry & Medical Genetics) en
dc.contributor.author Shields, Caroline
dc.date.accessioned 2010-09-10T22:00:25Z
dc.date.available 2010-09-10T22:00:25Z
dc.date.issued 2010-09-10T22:00:25Z
dc.identifier.uri http://hdl.handle.net/1993/4147
dc.description.abstract The purpose of this project was to determine how Dlc-2 and Rho signaling modulate the ceramide induction of PGP synthase. This induction was studied at the transcriptional, post-transcriptional, and post-translational levels using cell culture, Real-Time RT-PCR, protein purification, phage display, and western blotting techniques. We have demonstrated that the PGP synthase gene is not controlled at the transcriptional level by ceramide and Rho, nor is the mRNA stability of PGP synthase affected. However, ceramide and Rho do seem to exhibit translational or post-translational control over the PGP synthase protein. The relationships between Dlc-2 (and Rho), ceramide, and PGP synthase (and CL) are important to understand. All three are involved in cancer and apoptotic responses. The knowledge gained by the experiments discussed in this thesis will contribute to an understanding of how these proteins and lipids interact. This knowledge may then be used in the future to develop cancer treatments. en
dc.format.extent 5459123 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.rights info:eu-repo/semantics/openAccess
dc.subject Dlc-2 en
dc.subject PGP synthase en
dc.subject ceramide en
dc.subject cardiolipin en
dc.subject Rho en
dc.subject RhoGAP en
dc.title The role of Dlc-2 in ceramide signaling to PGP synthase en
dc.type info:eu-repo/semantics/masterThesis
dc.degree.discipline Biochemistry and Medical Genetics en
dc.contributor.examiningcommittee Davie, Jim (Biochemistry & Medical Genetics) Hatch, Grant (Pharmacology & Therapeutics) en
dc.degree.level Master of Science (M.Sc.) en
dc.description.note October 2010 en


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