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dc.contributor.supervisor Chelikani, Prashen (Oral Biology) en
dc.contributor.author Upadhyaya, Jasbir Deol
dc.date.accessioned 2010-09-10T16:12:57Z
dc.date.available 2010-09-10T16:12:57Z
dc.date.issued 2010-09-10T16:12:57Z
dc.identifier.citation Upadhyaya, Jasbir (2010) BBRC 398 (2) : 331-335 en
dc.identifier.uri http://hdl.handle.net/1993/4129
dc.description.abstract Bitter taste signaling in humans is mediated by a group of 25 bitter receptors (T2Rs) that belong to the G-protein coupled receptor (GPCR) family. Previously, several bitter peptides were isolated and characterized from bitter tasting food protein derived extracts, such as pea protein and soya bean extracts. However, their molecular targets in humans were poorly characterized. In this study, we tested the ability of the bitter tasting tri- and di-peptides to activate the human bitter receptor, T2R1. Using a heterologous expression system, T2R1 gene was transiently expressed in C6-glioma cells and changes in intracellular calcium were measured following addition of the peptides. We found that the bitter tasting tri-peptides are more potent in activating T2R1 than the di-peptides tested. Furthermore, to elucidate the potential ligand binding pocket of T2R1 we used homology molecular modeling. The ligand binding pocket in T2R1 is present on the extracellular surface of the receptor, and is formed by the transmembrane helices 1, 2, 3 and 7 and with extracellular loops 1 and 2. en
dc.format.extent 3586648 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.subject T2R1 en
dc.subject Peptides en
dc.subject Molecular modeling en
dc.subject C6 glial cells en
dc.title Characterization of human bitter taste receptor T2R1 en
dc.degree.discipline Oral Biology en
dc.contributor.examiningcommittee Parkinson, Fiona (Pharmacology and Therapeutics) Bhullar, Raj (Oral Biology) en
dc.degree.level Master of Science (M.Sc.) en
dc.description.note October 2010 en


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