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dc.contributor.supervisorKupriyanov, Valery V. (Biochemistry and Medical Genetics)en
dc.contributor.authorYang, Yanmin
dc.date.accessioned2010-07-08T22:16:20Z
dc.date.available2010-07-08T22:16:20Z
dc.date.issued2010-07-08T22:16:20Z
dc.identifier.citationYang Y, Sun J, Gervai P, Gruwel MLH, Jilkina O, Gussakovsky E, Yang X, Kupriyanov VV (2010). Characterization of Cryoinjury-Induced Infarction with Manganese- and Gadolinium-Enhanced MRI and Optical Spectroscopy in Pig Hearts. Magn Reson Imaging 28(5):753-766.en
dc.identifier.citationGussakovsky E, Yang Y (2010). On the Emission Intensity of Fluorescent Microspheres in Cardiac Tissue Images. J Fluoresc. Mar 9. [Epub ahead of print]en
dc.identifier.citationGruwel ML, Yang Y, Gervai P, Sun J, Kupriyanov V (2010). On the spatially specific and chronic delivery of growth hormones using alginate beads containing MR contrast agents (2009) Magn Reson Imaging 27(7):970-5en
dc.identifier.citationYang Y, Gruwel ML, Sun J, Gervai P, Yang X, Kupriyanov VV (2009). Manganese-enhanced MRI of acute cardiac ischemia and chronic infarction in pig hearts: kinetic analysis of enhancement development NMR Biomed 22(2):165-73en
dc.identifier.citationGussakovsky E, Jilkina O, Yang Y and Kupriyanov VV (2008) Hemoglobin plus myoglobin concentrations and near infrared light pathlength in phantom and pig hearts determined by diffuse reflectance spectroscopy. Anal Biochem 382(2):107-15en
dc.identifier.urihttp://hdl.handle.net/1993/4023
dc.description.abstractThe goals of the thesis were to, in pig models, (1) assess manganese-enhanced magnetic resonance imaging (MEMRI) in the characterization of acute and chronic ischemia-induced myocardial infarction (MI), (2) characterize cryoinjury-induced MI with contrast-enhanced MRI and optical methods, and (3) observe the effects of locally released angiogenic factors on the repair of cryoinjury-induced MI. Firstly, after acute MI was established by occlusion of the coronary artery branches, the pig hearts were isolated and mounted onto an ex vivo perfusion system inside a 7T magnet. After administration of MnCl2, T1-weighted MR images showed gradual enhancement of signal intensity within the normal myocardium, whereas the ischemic counterpart remained hypointense. During chronic MI progression, the intensity increased slowly after exposure to MnCl2 within the infarcted myocardium. Secondly, a new MI model was tested via direct 2-min contact of left ventricular epicardium with a liquid nitrogen-cooled aluminum bar. Subsequent in vivo Gd-enhanced MRI showed a uniform hypointense area (~10 mm in depth) surrounded by a hyperintense rim. Histology showed erythrocytes embolism within the cryolesion with a thin necrotic rim neighboring the normal myocardium. Four weeks later, the cryoinjured myocardium was replaced by scar tissue. Thirdly, in vivo MEMRI was tested on this cryoinjury model. After intravenous administration of MnCl2 via intermittent bolus or continuous infusion, normal myocardium showed prolonged hyperintense, which led to significant signal contrast between it and cryoinjured myocardium. Continuous infusion scheme minimized hemodynamic fluctuation. Finally, angiogenic therapy was assessed by anchoring of vascular growth factors-loaded alginate beads or adipose-derived stem cells (ADSCs)-loaded agarose patch on top of the cryoinjured myocardium. Gd-enhanced MRI revealed (1) growth of new tissue wrapping the growth factors-loaded alginate beads and (2) higher perfusion within the ADSCs-treated cryoinjured myocardium as compared with the growth factors-treated counterpart. Histological and fluorescent microsphere examination revealed that ADSCs induced more significant growth of mature microvasculature within the cryoinjured myocardium. These results indicate that MnCl2 could characterize MI ex vivo and in vivo. Epicardial implantation of ADSCs-loaded agarose hydrogel can induce angiogenesis within the cryoinjured myocardium, a form of MI with similar progression features as that induced by ischemia.en
dc.format.extent3327124 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMyocardial infarctionen
dc.subjectMagnetic resonance imagingen
dc.subjectNear infrared spectroscopic imagingen
dc.subjectManganese chlorideen
dc.subjectAngiogenic factorsen
dc.subjectAdipose-derived stem cellsen
dc.titleCharacterization of myocardial infarction and its repair in pig models using MRI and optical spectroscopy and imagingen
dc.typeinfo:eu-repo/semantics/doctoralThesis
dc.typedoctoral thesisen_US
dc.degree.disciplineBiochemistry and Medical Geneticsen_US
dc.contributor.examiningcommitteeWrogemann, Klaus (Biochemistry and Medical Genetics) Yang, Xi (Medical Microbiology) Tian, Ganghong (Physiology) Wright, Graham A. (Sunnybrook Health Sciences Centre)en
dc.degree.levelDoctor of Philosophy (Ph.D.)en_US
dc.description.noteOctober 2010en


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