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dc.contributor.supervisor Fouad Daayf (Department of Plant Science) Khalid Y. Rashid (Cereal Research Centre, Agriculture and Agri-Food Canada) en
dc.contributor.author YAO, ZHEN
dc.date.accessioned 2009-12-23T14:42:48Z
dc.date.available 2009-12-23T14:42:48Z
dc.date.issued 2009-12-23T14:42:48Z
dc.identifier.uri http://hdl.handle.net/1993/3838
dc.description.abstract Verticillium wilt, caused by the soil-borne Verticillium dahliae Klebahn is a serious problem in the production of sunflower worldwide. To date, information on sunflower resistance to Verticillium spp. is very scarce, although it is critical for an effective management of this pathogen. In this study, two highly aggressive (Vd1396-9 and Vd1398-21) and two weakly aggressive V. dahliae isolates (Vs06-07 and Vs06-14) were used to inoculate moderately resistant (IS6111) and susceptible (IS8048) sunflower hybrids. VdNEP (V. dahliae necrosis and ethylene-inducing protein), an elicitor from V. dahliae, was also used to infiltrate sunflower plants. Our results indicate that VdNEP has a dual role in the interaction between sunflower and V. dahliae. VdNEP acted not only as a pathogenicity factor on sunflower by inducing wilting symptoms such as chlorosis, necrosis and vascular discoloration, but also as an elicitor triggering defense responses of the host. VdNEP induced the hypersensitive cell death in Nicotiana benthamiana leaves and sunflower cotyledons. Moreover, VdNEP activated the production of reactive oxygen species and the accumulation of fluorescent compounds in sunflower leaves. Pathogenesis-related genes (Ha-PR-3, and Ha-PR-5), two defensin genes (Ha-PDF and Ha-CUA1) and genes encoding Ha-ACO, Ha-CHOX, Ha-GST and Ha-SCO were up-regulated by VdNEP, suggesting that multiple signaling pathways are involved in this interaction. Two SA-related genes (Ha-PAL and Ha-NML1) were slightly suppressed after infiltration with VdNEP, suggesting a possible involvement of VdNEP in affecting sunflower defenses. en
dc.format.extent 1330392 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.subject Verticillium wilt en
dc.subject Sunflower en
dc.subject VdNEP en
dc.subject Pathogenicity en
dc.subject Elicitor en
dc.subject Plant defense response en
dc.title MOLECULAR CHARACTERIZATION OF THE INTERACTION BETWEEN HELIANTHUS ANNUUS AND VERTICILLIUM DAHLIAE en
dc.degree.discipline Plant Science en
dc.contributor.examiningcommittee Dana Schroeder (Departement of microbiology) Muhammad Tahir (Department of Plant Science) en
dc.degree.level Master of Science (M.Sc.) en
dc.description.note February 2010 en


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