The influence of plant and animal hormones on growth and accumulation of pigments and fatty acids in the microalgae Scenedesmus quadricauda (CPCC-158) and the duckweed Lemna minor (CPCC-490).

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Date
2019
Authors
Kozlova, Tatiana
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Abstract
The production of biofuels and high-valuae biochemical commodities from microalgae may be considered “third generation biofuels”. Interest in the use of wastewaters for the industrial production of microalgae for biofuels and bioproducts has stimulated the studies on the influence of a variety of compounds in wastewaters on microalgae physiology. In this study the influence of four phytohormones (brassinolide, BL; 24-epi-brassinolide, EBL; 3-indole acetic acid, IAA, and abscisic acid, ABB) singularly and in some combinations, and two animal sex hormones (17β-estradiol, E2 and 17, 20β-dihydroxy-4-pregnen-3-one, 17,20β-P), on the physiology of Scenedesmus quadricauda was tested. The influence of 17β-estradiol on physiology of S. quadricauda and the Lemna minor grown in three different media (Bolt Basal Medium, natural Fishery Wastewater, and Reconstituted Fishery Wastewater) was also investigated. All the hormones tested had positive stimulatory effects on the growth and biosynthetic activity of S. quadricauda. EBL and IAA most induced pigments accumulation while the effect on fatty acids accumulation followed the order of: IAA ≥ ABA > EBL > BL. Both the quantities of fatty acids and their profiles depended on the hormone type and the specific concentrations tested. Synergistic increases in S. quadricauda biomass production, pigments accumulation, and neutral lipid accumulation were observed when EBL and IAA were assessed in combined-hormones trials. The stimulatory effect was 1.7-, 2.7-, and 3.3-fold greater for chlorophyll-a, total carotenoids, and fatty acids, respectively compared with single hormone treatments. Both animal steroids tested, E2 and 17,20β-P, demonstrated positive stimulatory effects on S. quadricauda cell growth parameters, pigments, and lipid accumulation. A comparison of E2 performance in three types of growth media (BBM, FWW and RFWW) revealed differences in the ability of E2 to induce growth and biosynthetic activities of both S. quadricauda and L. minor. The most effective E2 concentrations were also evidently different for the algae and the plant when they were grown in the same type of medium. Our results highlight the possibility that hormones could be used as a tool for algal biosynthesis manipulation in the biofuel, pharmaceutical and cosmetic industries.
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Keywords
Phytohormones, Fish steroids, Microalgae, Chlorophyll-a, Carotenoid, Lipids
Citation
Tatiana A. Kozlova, Bruce P. Hardy, Priti Krishna, David B. Levin, 2017. Effect of phytohormones on growth and accumulation of pigments and fatty acids in the microalgae Scenedesmus quadricauda, Algal Research 27: 325-334.
Tatiana A. Kozlova, Bruce P. Hardy, David B. Levin, 2018. The combined influence of 24‑epibrassinolide and 3‑indoleacetic acid on growth and accumulation of pigments and fatty acids in the microalgae Scenedesmus quadricauda (CPCC-158), Algal Research 35: 22-32.
Tatiana A. Kozlova, Bruce P. Hardy, David B. Levin, 2019. Effect of Fish Steroids 17β-estradiol and 17,20β-dihydroxy-4-pregnen-3-one on Growth, Accumulation of Pigments, and Fatty Acid Profiles in the Microalgae Scenedesmus quadricauda (CPCC-158), Renewable Energy (RENE-S-19-02346-2 in press).