Properties of layer-by-layer single-cell encapsulation and the potential applications in transplantation therapy for neurologic disorders
Loading...
Date
2018-12-05
Authors
Li, Wenyan
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Layer-by-layer (LbL) self-assembly is a rapidly developing biomaterial technique for fabricating multilayered structure on substrates. In this project, we report the encapsulation of single neural stem cells (NSCs) using the LbL self-assembly technique with gelatin and alginate. The encapsulation did not affect normal functions of the encapsulated NSCs. When insulin-like growth factor-1 was loaded on materials, its release significantly enhanced the proliferation of the encapsulated NSCs.
Permeability of the LbL encapsulation was investigated on PC12 cells encapsulated with gelatin and hyaluronic acid (HA). The permeability was inversely proportional to molecular weight of FITC-dextran (FD), material concentration, and number of layers. Encapsulation with 0.5% of the materials for 8 layers was proven to effectively inhibit the cell apoptosis induced by tumor necrosis factor-α (TNF-α).
Since the encapsulation structure creates a favorable microenvironment for cells to survive and function, it holds significant potential for cell therapies for neurologic disorders.
Description
Keywords
Layer-by-layer, Cell encapsulation, Drug delivery, Permeability, Microenvironment regulation
Citation
Wenyan Li, Guohui Zhang, Teng Guan, Xiaosha Zhang, Ali Khosrozadeh, Malcolm Xing* and Jiming Kong*. Manipulable Permeability of Nanogel Encapsulation on Cells Exerts Protective Effect against TNF-α-Induced Apoptosis. ACS Biomater. Sci. Eng. 2018, 4, 2825−2835.
Wenyan Li , Teng Guan , Xiaosha Zhang , Ziyuan Wang , Meng Wang , Wen Zhong , Hua Feng , Malcolm M.Q. Xing* , and Jiming Kong*. The effect of layer-by-layer assembly coating on the proliferation and differentiation of neural stem cells. ACS Applied Materials & Interfaces, 2015, 7(5), 3018-3029.
Shilun Zuo, Wenyan Li, Qiang Li, Hengli Zhao, Jun Tang, Qianwei Chen, Xin Liu, John H. Zhang, Yujie Chen* and Hua Feng*. Protective effects of Ephedra sinica extract on blood– brain barrier integrity and neurological function correlate with complement C3 reduction after subarachnoid hemorrhage in rats. Neuroscience Letters, 2015, 609, 216-222.
Shuhan Liu, Alex C.-T. Ko, Wenyan Li, Wen Zhong* and Malcolm Xing*. NIR initiated and pH sensitive single-wall carbon nanotubes for doxorubicin intracellular delivery. Journal of Materials Chemistry B, 2014, 2, 1125-1135.
Chengren Li, Teng Guan, Xueping Chen, Wenyan Li, Qiyan Cai, Jianqin Niu, Lan Xiao* and Jiming Kong*. BNIP3 mediates pre-myelinating oligodendrocyte cell death in hypoxia and ischemia. Journal of Neurochemistry, 2013, 127, 426-433.
Wenyan Li , Teng Guan , Xiaosha Zhang , Ziyuan Wang , Meng Wang , Wen Zhong , Hua Feng , Malcolm M.Q. Xing* , and Jiming Kong*. The effect of layer-by-layer assembly coating on the proliferation and differentiation of neural stem cells. ACS Applied Materials & Interfaces, 2015, 7(5), 3018-3029.
Shilun Zuo, Wenyan Li, Qiang Li, Hengli Zhao, Jun Tang, Qianwei Chen, Xin Liu, John H. Zhang, Yujie Chen* and Hua Feng*. Protective effects of Ephedra sinica extract on blood– brain barrier integrity and neurological function correlate with complement C3 reduction after subarachnoid hemorrhage in rats. Neuroscience Letters, 2015, 609, 216-222.
Shuhan Liu, Alex C.-T. Ko, Wenyan Li, Wen Zhong* and Malcolm Xing*. NIR initiated and pH sensitive single-wall carbon nanotubes for doxorubicin intracellular delivery. Journal of Materials Chemistry B, 2014, 2, 1125-1135.
Chengren Li, Teng Guan, Xueping Chen, Wenyan Li, Qiyan Cai, Jianqin Niu, Lan Xiao* and Jiming Kong*. BNIP3 mediates pre-myelinating oligodendrocyte cell death in hypoxia and ischemia. Journal of Neurochemistry, 2013, 127, 426-433.