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dc.contributor.author Chatterjee, Sumanta
dc.contributor.author Basak, Pratima
dc.contributor.author Buchel, Edward
dc.contributor.author Murphy, Leigh C
dc.contributor.author Raouf, Afshin
dc.date.accessioned 2018-11-01T13:53:24Z
dc.date.issued 2018-10-04
dc.identifier.citation Stem Cell Research & Therapy. 2018 Oct 04;9(1):264
dc.identifier.uri https://doi.org/10.1186/s13287-018-0994-y
dc.identifier.uri http://hdl.handle.net/1993/33547
dc.description.abstract Abstract Background Normal human breast epithelial cells are maintained by the proliferation and differentiation of different human breast epithelial progenitors (HBEPs). However, these progenitor subsets can only be obtained at low frequencies, limiting their further characterization. Recently, it was reported that HBEPs can be minimally expanded in Matrigel cocultures with stromal feeder cells. However, variability of generating healthy feeder cells significantly impacts the effective expansion of HBEPs. Methods Here, we report a robust feeder cell-free culture system for large-scale expansion of HBEPs in two-dimensional cultures. Results Using this cell culture system HBEPs can be exponentially expanded as bulk cultures. Moreover, purified HBEP subtypes can also be separately expanded using our cell culture system. The expanded HBEPs retain their undifferentiated phenotype and form distinct epithelial colonies in colony forming cell assays. Conclusions The availability of a culture system enabling the large-scale expansion of HBEPs facilitates their application to screening platforms and other cell-based assays.
dc.rights info:eu-repo/semantics/openAccess
dc.title A robust cell culture system for large scale feeder cell-free expansion of human breast epithelial progenitors
dc.type Journal Article
dc.type info:eu-repo/semantics/article
dc.language.rfc3066 en
dc.rights.holder The Author(s).
dc.date.updated 2018-11-01T13:53:24Z


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