Pseudomonas chlororaphis strain PA23 plant growth promotion characteristics and product formulation development.
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Date
2018-06
Authors
Amanda Holden
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Abstract
Pseudomonas chlororaphis PA23 is a biocontrol agent that exhibits plant growth promotion (PGP) potential. The focus of this research was to investigate the PGP capacity of PA23 either through drenching soil-grown plants with bacterial suspensions or by exposing plants to bacterial volatiles in enclosed split-plate assays. In soil experiments, canola and lettuce treated with PA23 and the gacS mutant showed no increase in biomass. However, plants exposed to bacterial volatiles were significantly larger. Liquid- and dry-based formulations were investigated for their impact on PA23 cell viability over the course of 1 year at 4oC. Liquid formulations contained PA23 cells suspended in LB with one or more additives to protect against desiccation. For dry formulations, PA23 was cultured in nutritional, minimal or high salt media and lyophilized with lactose. While dry formulations maintained a relatively high viability over time, they were unable to protect canola from infection with Leptosphaeria maculans. Liquid formulations demonstrated better biocontrol properties but showed a dramatic loss of viability upon storage at 4oC.
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Keywords
Plant growth promotion, Storage formulation, Organic volatile compounds, PA23
Citation
ASA