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dc.contributor.supervisor Karimi-Abdolrezaee, Soheila (Physiology and Pathophysiology) en_US
dc.contributor.author Mohammadzadeh Shahriary, Ghazaleh
dc.date.accessioned 2018-08-15T15:31:14Z
dc.date.available 2018-08-15T15:31:14Z
dc.date.issued 2018-08-04 en_US
dc.date.submitted 2018-08-05T02:39:04Z en
dc.identifier.uri http://hdl.handle.net/1993/33201
dc.description.abstract Microglia are resident immune cells of the central nervous system, which in concert with astrocytes and peripherally recruited immune cells initiate a pro-inflammatory response after injury and disease that results in oligodendroglial death and myelin damage. We have shown that down-regulation of a neuronally-derived growth factor, neuregulin-1, in demyelinating lesions of the spinal cord is an underlying mechanism for insufficient spontaneous oligodendrogenesis and remyelination. Recent evidence suggests that Nrg-1 treatment positively regulates the repair process and remyelination by modulating neuroinflammation. The goal of the present study was to determine the role of Nrg-1 in regulating microglia response in normal and injury state. In primary in vitro systems, we demonstrate a positive role for Nrg-1 in regulating microglia activity and the impact of Nrg-1 treatment on the effects of microglia on the behavior of neural precursor cells (NPCs). Using an array of cellular and molecular assays, we found that Nrg-1 attenuated the transcript expression of several pro-inflammatory markers such as tumor necrosis factor- (TNF-) α, interleukin- (IL-) 6, CD86 and the production of nitric oxide (NO). In addition, Nrg-1 restored the suppressed phagocytic ability in M1-polarized microglia cultures. Our findings showed that microglia conditioned media (MCM) from Nrg-1 treated M1-polarized microglia cultures promoted migration and proliferation of NPCs. Hence, our findings suggest that Nrg-1 therapy could be exploited to foster a pro-regenerative phenotype in microglia, which is supportive of repair and regeneration following CNS injuries and diseases. en_US
dc.subject Neuregulin-1 en_US
dc.subject Microglia en_US
dc.subject In vitro studies en_US
dc.subject NPCs en_US
dc.title Uncovering the role of neuregulin-1 in regulating microglia properties: in vitro studies en_US
dc.degree.discipline Physiology and Pathophysiology en_US
dc.contributor.examiningcommittee Dhingra, Sanjiv (Physiology and Pathophysiology) Eftekharpour, Eftekhar (Physiology and Pathophysiology) Kauppinen, Tiina (Pharmacology and Therapeutics) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note October 2018 en_US


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