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dc.contributor.supervisorGordon, Richard (Electrical and Computer Engineering) Thomas, Gabriel (Electrical and Computer Engineering)en
dc.contributor.authorCrawford-Young, Susan J.
dc.date.accessioned2007-04-27T14:20:48Z
dc.date.available2007-04-27T14:20:48Z
dc.date.issued2007-04-27T14:20:48Z
dc.identifier.urihttp://hdl.handle.net/1993/331
dc.description.abstractA robotic microscope was designed using a microcontroller to take time-lapse digital photographs of developing salamander embryos. The microcontroller operated three stepper motors to control three-axis movement accurately, and two six mega-pixel digital cameras to capture through-focus time-lapse digital pictures of six views of Ambystoma mexicanum embryos (axolotl, a salamander). The device is designed to take images every five minutes for 80 hours of early development, from fertilization to stage 20, when the neural tube closes to form the brain and spinal column. Techniques to enhance the embryo images were investigated including image fusion to get in-focus views from a stack of images. In the early embryo surface epithelial cells differentiate to form neural tissue and external skin tissue. Observing the whole embryo surface at cellular level will give a better idea of the stress and strain each cell undergoes and what physical forces are involved in cell differentiation.en
dc.format.extent79037982 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjecttime-lapseen
dc.subject3D imagingen
dc.subjectembryoen
dc.subjectdevelopmenten
dc.subjecturodeleen
dc.subjectsalamanderen
dc.subjectaxolotlen
dc.subjectAmbystoma mexicanumen
dc.subjectroboticen
dc.subjectmicroscopeen
dc.titleA robotic microscope for 3D time-lapse imaging of early stage axolotl salamander embryosen
dc.typeinfo:eu-repo/semantics/masterThesis
dc.typemaster thesisen_US
dc.degree.disciplineElectrical and Computer Engineeringen_US
dc.contributor.examiningcommitteeCicek, Nazim (Biosystems Engineering) Ferens, Ken (Electrical and Computer Engineering)en
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.noteMay 2007en


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