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dc.contributor.supervisor Gordon, Richard (Electrical and Computer Engineering) Thomas, Gabriel (Electrical and Computer Engineering) en
dc.contributor.author Crawford-Young, Susan J.
dc.date.accessioned 2007-04-27T14:20:48Z
dc.date.available 2007-04-27T14:20:48Z
dc.date.issued 2007-04-27T14:20:48Z
dc.identifier.uri http://hdl.handle.net/1993/331
dc.description.abstract A robotic microscope was designed using a microcontroller to take time-lapse digital photographs of developing salamander embryos. The microcontroller operated three stepper motors to control three-axis movement accurately, and two six mega-pixel digital cameras to capture through-focus time-lapse digital pictures of six views of Ambystoma mexicanum embryos (axolotl, a salamander). The device is designed to take images every five minutes for 80 hours of early development, from fertilization to stage 20, when the neural tube closes to form the brain and spinal column. Techniques to enhance the embryo images were investigated including image fusion to get in-focus views from a stack of images. In the early embryo surface epithelial cells differentiate to form neural tissue and external skin tissue. Observing the whole embryo surface at cellular level will give a better idea of the stress and strain each cell undergoes and what physical forces are involved in cell differentiation. en
dc.format.extent 79037982 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.subject time-lapse en
dc.subject 3D imaging en
dc.subject embryo en
dc.subject development en
dc.subject urodele en
dc.subject salamander en
dc.subject axolotl en
dc.subject Ambystoma mexicanum en
dc.subject robotic en
dc.subject microscope en
dc.title A robotic microscope for 3D time-lapse imaging of early stage axolotl salamander embryos en
dc.degree.discipline Electrical and Computer Engineering en
dc.contributor.examiningcommittee Cicek, Nazim (Biosystems Engineering) Ferens, Ken (Electrical and Computer Engineering) en
dc.degree.level Master of Science (M.Sc.) en
dc.description.note May 2007 en


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