MSpace will be unavailable on Sunday Jan 29, 2023 between the times of 8:00AM CST and 12:00PM CST. Please plan your submissions accordingly.

Show simple item record

dc.contributor.supervisorEck, Peter (Human Nutritional Sciences)en_US
dc.contributor.authorAlharbi, REHAB
dc.description.abstractThe solute carrier SLC23A1, encoded by the SLC23A1 gene, is the key membrane transporter mediating the reabsorption of ascorbic acid, the reduced form of vitamin C, in the renal proximal tubule. It had been shown that a single nucleotide polymorphism (SNP) in SLC23A1 could alter the proteins ability to transport ascorbic acid. Recently, through various human re-sequencing projects, many novel single nucleotide variations (SNV) have been discovered and their information has been deposited in genomic databases. However, their impact on the proteins functions remains to be determined. In a first step for functional studies, SNV need to be introduced into an existing SLC23A1 expression plasmids. The objective of this study was to introduce selected SNVs into the coding region, or Open Reading Frame (ORF), of the SLC23A1 expression plasmid pReceiver-M55-SLC23A1. Six SNVs located proximal to the 5’ and 3’ ends of the SLC23A1 ORF were successfully introduced.en_US
dc.titleIntroducing Single Nucleotide variations into the Vitamin C Transporter SLC23Aen_US
dc.typemaster thesisen_US Nutritional Sciencesen_US
dc.contributor.examiningcommitteeFreil, James (Human Nutritional Sciences) Weihrauch, Dirk (Biological Science)en_US of Science (M.Sc.)en_US
dc.description.noteFebruary 2017en_US

Files in this item


This item appears in the following Collection(s)

Show simple item record