Exploitation of human RuvBL1 by HAdV E1A to inhibit interferon response for efficient viral growth
Loading...
Date
2016
Authors
Olanubi, Oladunni
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
E1A of human adenovirus is the first gene product expressed during viral infection and serves as a preliminary step for efficient viral replication. Other early gene products include early proteins designated as E2, E3, and E4 are also made, which together with E1A prepare the infected cell for replication of the viral genome. Various studies have elucidated that E1A functions largely as a transcriptional regulator and can interact with a host of cellular modulators to enhance replication of the virus. RuvBL1, a chromatin remodeling protein involved in a host of cellular functions such as transcriptional regulation and host cell immune response has been shown to bind to E1A. My results identify RuvBL1 as an E1A binding protein and show that E1A is a direct binding partner of RuvBL1. I demonstrate that RuvBL1 plays a role in the growth of adenovirus, as knockdown of RuvBL1 negatively affects the growth of the virus and reveals that RuvBL1 functions as a viral growth enhancer. Finally, I identify a possible role of E1A’s inhibition of interferon response in a RuvBL1 dependent manner.
Description
Keywords
Adenovirus