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Development of a targeted proteomic assay for rapid detection of Shiga-like toxins 1 and 2 in Shiga toxin-producing Escherichia coli

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dc.contributor.supervisor Graham, Morag (Medical Microbiology and Infectious Diseases) en_US
dc.contributor.author Scharikow, Leanne Gene
dc.date.accessioned 2017-01-05T21:33:21Z
dc.date.available 2017-01-05T21:33:21Z
dc.date.issued 2016
dc.identifier.uri http://hdl.handle.net/1993/31991
dc.description.abstract Shiga toxin-producing Escherichia coli (STEC) are extensive contributors to foodborne illness, causing renal and central nervous system damage due to production of Shiga toxin (Stx). Rapid Stx detection is important to distinguish STEC from other enteric pathogens. Current detection techniques are time consuming, expensive, and lack sensitivity. We have developed and evaluated a novel targeted mass spectrometry-based assay for detection of Stx using parallel reaction monitoring (PRM). The PRM assay used 11 target tryptic peptides and was validated using STEC and non-STEC bacterial cultures. Stx was detected in 56 of 62 STEC isolates and did not detect Stx in any of the 29 non-STEC isolates. The PRM assay successfully determined the Stx2 subtype in 32 of 46 Stx2-positive isolates. By applying a targeted proteomics assay, we were able to simultaneously detect Stx toxins 1 and 2 and subtype Stx2 into six toxin subgroups in Stx2-positive isolates. en_US
dc.subject Mass spectrometry en_US
dc.subject Targeted proteomics en_US
dc.subject Shiga toxin-producing Escherichia coli en_US
dc.subject Shiga toxin en_US
dc.title Development of a targeted proteomic assay for rapid detection of Shiga-like toxins 1 and 2 in Shiga toxin-producing Escherichia coli en_US
dc.degree.discipline Medical Microbiology en_US
dc.contributor.examiningcommittee McClarty, Grant (Medical Microbiology and Infectious Diseases) Nadon, Celine (Medical Microbiology and Infectious Diseases) Narvaez, Claudia (Food Science) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note February 2017 en_US


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