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dc.contributor.supervisor Bridges, Greg (Electrical and Computer Engineering) en_US
dc.contributor.author Salimi, Elham
dc.date.accessioned 2016-12-06T20:34:15Z
dc.date.available 2016-12-06T20:34:15Z
dc.date.issued 2016-01 en_US
dc.identifier.citation E. Salimi, and D.J. Thomson, G.E. Bridges, " Dielectric model for Chinese hamster ovary cells obtained by dielectrophoresis cytometry", Biomicrofluidics, vol. 10, no. 1, pp. 014111, 2016 en_US
dc.identifier.uri http://hdl.handle.net/1993/31945
dc.description.abstract Electroporation affects the dielectric properties of cells. Dielectric measurement techniques can provide a label-free and non-invasive modality to study this phenomenon. In this thesis we introduce a dielectrophoresis (DEP) based technique to study changes in the cytoplasm conductivity of single Chinese hamster ovary (CHO) cells immediately after electroporation. Using a microfluidic chip, we study changes in the DEP response of single CHO cells a few seconds after electroporation. First, in order to quantify our DEP measurement results and relate them to the cells internal conductivity, we introduce a dielectric model for CHO cells. This is achieved by measuring the DEP response of many individual cells in the β-dispersion frequency region and curve fitting to the measured data. Second, we present quantitative results for changes in the cytoplasm conductivity of single cells subjected to pulsed electric fields with various intensities. We observe that when electroporation is performed in media with lower ionic concentration than cells cytoplasm, their internal conductivity decreases after electroporation depending on the intensity of applied pulses. We also observe that with reversible electroporation there is a limit on the decrease in the cells’ internal conductivity. We hypothesize the reason is the presence of large and relatively immobile negative ions inside the cell which attract mobile positive ions (mainly sodium and potassium) to maintain cell electrical neutrality. We monitor the temporal response of cells after electroporation to measure the time constant of changes due to ion transport and observe this ranges from seconds to tens of seconds depending on the applied pulse intensity. This result can be used to infer information about the density and resealing time of very small pores (not measurable with conventional marker molecules). Lastly, we measure the electroporation of cells in media with different conductivities. Our results show that electroporation in very low conductivity media requires stronger pulses to achieve a similar poration extent as in high conductivity media. The outcome of this thesis can be used to improve our understanding of the dynamics of electroporation as well as its modelling in order to make more accurate predictions or optimize the process for specific applications. en_US
dc.publisher American Institute of Physics en_US
dc.subject Dielectrophoresis en_US
dc.subject Electroporation en_US
dc.subject Chinese hamster ovary cells en_US
dc.subject CHO en_US
dc.subject Dielectric properties en_US
dc.subject Microwave interferometer en_US
dc.subject Pulsed electric field en_US
dc.subject Cytoplasm conductivity en_US
dc.subject Single cell en_US
dc.title Dielectrophoresis study of electroporation effects on dielectric properties of biological cells en_US
dc.degree.discipline Electrical and Computer Engineering en_US
dc.contributor.examiningcommittee Thomson, Douglas (Electrical and Computer Engineering) Lin, Francis (Physics and Astronomy) Wang, Pingshan (Electrical and Computer Engineering, Clemson University) en_US
dc.degree.level Doctor of Philosophy (Ph.D.) en_US
dc.description.note February 2017 en_US


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