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dc.contributor.author Li, Wei
dc.contributor.author Zhang, Jiefu
dc.contributor.author Mou, Yanglong
dc.contributor.author Geng, Jianfeng
dc.contributor.author McVetty, Peter B
dc.contributor.author Hu, Shengwu
dc.contributor.author Li, Genyi
dc.date.accessioned 2016-11-23T15:00:41Z
dc.date.available 2016-11-23T15:00:41Z
dc.date.issued 2011-05-19
dc.identifier.citation BMC Genomics. 2011 May 19;12(1):249
dc.identifier.uri http://dx.doi.org/10.1186/1471-2164-12-249
dc.identifier.uri http://hdl.handle.net/1993/31936
dc.description.abstract Abstract Background Sequence related amplified polymorphism (SRAP) is commonly used to construct high density genetic maps, map genes and QTL of important agronomic traits in crops and perform genetic diversity analysis without knowing sequence information. To combine next generation sequencing technology with SRAP, Illumina's Solexa sequencing was used to sequence tagged SRAP PCR products. Results Three sets of SRAP primers and three sets of tagging primers were used in 77,568 SRAP PCR reactions and the same number of tagging PCR reactions respectively to produce a pooled sample for Illumina's Solexa sequencing. After sequencing, 1.28 GB of sequence with over 13 million paired-end sequences was obtained and used to match Solexa sequences with their corresponding SRAP markers and to integrate Solexa sequences on an ultradense genetic map. The ultradense genetic bin map with 465 bins was constructed using a recombinant inbred (RI) line mapping population in B. rapa. For this ultradense genetic bin map, 9,177 SRAP markers, 1,737 integrated unique Solexa paired-end sequences and 46 SSR markers representing 10,960 independent genetic loci were assembled and 141 unique Solexa paired-end sequences were matched with their corresponding SRAP markers. The genetic map in B. rapa was aligned with the previous ultradense genetic map in B. napus through common SRAP markers in these two species. Additionally, SSR markers were used to perform alignment of the current genetic map with other five genetic maps in B. rapa and B. napus. Conclusion We used SRAP to construct an ultradense genetic map with 10,960 independent genetic loci in B. rapa that is the most saturated genetic map ever constructed in this species. Using next generation sequencing, we integrated 1,878 Solexa sequences on the genetic map. These integrated sequences will be used to assemble the scaffolds in the B. rapa genome. Additionally, this genetic map may be used for gene cloning and marker development in B. rapa and B. napus.
dc.title Integration of Solexa sequences on an ultradense genetic map in Brassica rapa L.
dc.type Journal Article
dc.language.rfc3066 en
dc.rights.holder Li et al; licensee BioMed Central Ltd.
dc.date.updated 2016-11-22T17:09:17Z


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