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Prostate cancer circulating tumor cells: automated and manual enumeration after isolation via size-based filtration of pre-treatment patient samples.

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dc.contributor.supervisor Mai, Sabine (Human Anatomy and Cell Science) en_US
dc.contributor.author Alsaadi, Hazem
dc.date.accessioned 2016-10-05T16:14:46Z
dc.date.available 2016-10-05T16:14:46Z
dc.date.issued 2016
dc.identifier.uri http://hdl.handle.net/1993/31878
dc.description.abstract CTCs have emerged as a potential source of clinical significance. But with numerous isolating systems currently available, the numbers of captured CTCs vary widely. At this point, CellSearch remains the only FDA-approved system with clinical significance whereby the results could be used to monitor patients with metastatic colon, breast, or prostate cancer. However, its inability to isolate CTCs from non-high risk prostate cancer patients or CTCs that are EpCAM-negative has led to criticism. In this study, we have shown that size-based filtration successfully isolates CTCs from patients with localized and metastatic prostate cancer. We have also shown that CTCs can be successfully isolated from low and intermediate risk groups. Additionally, clusters of CTCs were preserved and isolated in all localized risk groups and metastatic patients. Furthermore, we enumerated the isolated CTCs using automated and manual methods in low risk, intermediate risk, high risk, and metastatic prostate cancer. The automated and manual counts were comparable. Moreover, the amounts of clusters and the size of clusters correlated with the status and stage of prostate cancer. en_US
dc.subject Circulating Tumor Cells, Prostate Cancer, Filtration, ScreenCell, Androgen Receptor, Enumeration en_US
dc.title Prostate cancer circulating tumor cells: automated and manual enumeration after isolation via size-based filtration of pre-treatment patient samples. en_US
dc.degree.discipline Human Anatomy and Cell Science en_US
dc.contributor.examiningcommittee Klonisch, Thomas (Human Anatomy and Cell Science) Drachenberg, Darrel (Surgery-Urology) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note October 2016 en_US


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