Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada
Loading...
Date
2016-08
Authors
Fernando, Dinesh M.
Tun, Hein Min
Poole, Jenna
Patidar, Rakesh
Li, Ru
Mi, Ruidong
Amarawansha, Geethani E. A.
Fernando, W. G. Dilantha
Khafipour, Ehsan
Farenhorst, Annemieke
Journal Title
Journal ISSN
Volume Title
Publisher
American Society for Microbiology
Abstract
Access to safe drinking water is now recognized as a human right by the United Nations. In developed countries like Canada, access to clean water is generally not a matter of concern. However, one in every five First Nations reserves is under a drinking water advisory, often due to unacceptable microbiological quality. In this study, we analyzed source and potable water from a First Nations community for the presence of coliform bacteria as well as various antibiotic resistance genes. Samples, including those from drinking water sources, were found to be positive for various antibiotic resistance genes, namely, ampC, tet(A), mecA, -lactamase genes (SHV-type, TEM-type, CTX-M-type, OXA-1, and CMY-2-type), and carbapenemase genes (KPC, IMP, VIM, NDM, GES, and OXA-48 genes). Not surprisingly, substantial numbers of total coliforms, including Escherichia coli, were recovered from these samples, and this result was also confirmed using Illumina sequencing of the 16S rRNA gene. These findings deserve further attention, as the presence of coliforms and antibiotic resistance genes potentially puts the health of the community members at risk.
Description
Keywords
drinking water, human right, antibiotic resistance, coliforms, Illumina sequencing, First Nations reserves, drinking water advisory, microbiological quality
Citation
Fernando, D.M., Tun, H.M., Poole, J., Patidar, R., LI, R., MI, R., Amarawansha, G.E.A., Fernando, W.G.D., Khafipour, E., Farenhorst, A., Kumar, A. (2016). Detection of antibiotic resistance genes in source and drinking water samples from a First Nation Community in Canada. Applied and Environmental Microbiology. 82(15): 4767-4775.