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dc.contributor.supervisor Albensi, Benedict (Pharmacology And Therapeutics) Portet, Stephanie (Mathematics) en_US
dc.contributor.author Cadonic, Chris
dc.date.accessioned 2016-08-24T21:36:51Z
dc.date.available 2016-08-24T21:36:51Z
dc.date.issued 2016
dc.identifier.uri http://hdl.handle.net/1993/31602
dc.description.abstract A computational model for mitochondrial function has been developed from oxygen concentration data measured in the Oroboros Oxygraph-2k and oxygen consumption rates measured in the Seahorse XF24 Analyzer. Measurements were acquired using embryonic-cultured cortical neurons and isolated mitochondria from CD1 mice. Based on the biological mechanism of mitochondrial activity, a computational model was developed using biochemical kinetic modelling. To modulate mitochondrial activity, dysfunctions were introduced by injecting the inhibiting reagents oligomycin, rotenone, and antimycin A, and the uncoupling reagent carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) during measurements. To incorporate these changes, model equations were adapted and globally calibrated to experimental data using the genetic algorithm developed by Jason Fiege of the University of Manitoba by fitting oxygen concentration data. The model was coded in MATLAB R2014a along with the development of a graphical user interface for simulating mitochondrial bioenergetics in silico. en_US
dc.subject Mathematical Model en_US
dc.subject Computational Model en_US
dc.subject Mitochondria en_US
dc.subject Bioenergetics en_US
dc.subject Kinetics en_US
dc.subject Oroboros Oxygraph-2k en_US
dc.subject Complex IV en_US
dc.title Modelling mitochondrial complex IV bioenergetics en_US
dc.degree.discipline Biomedical Engineering en_US
dc.contributor.examiningcommittee Thomson, Douglas (Electrical & Computer Engineering) Amara, Francis (Biochemistry & Medical Genetics) Treberg, Jason (Biological Sciences) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note October 2016 en_US


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