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Evaluation of the Anti-Inflammatory Effects of Creatine in Canine Chondrocytes as an in-vitro Model of Joint Inflammation

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dc.contributor.supervisor Miller, Donald (Pharmacology and Therapeutics) en_US
dc.contributor.author Alraddadi, Eman
dc.date.accessioned 2016-04-07T15:45:04Z
dc.date.available 2016-04-07T15:45:04Z
dc.date.issued 2016
dc.identifier.uri http://hdl.handle.net/1993/31184
dc.description.abstract Little is known about the anti-inflammatory activity of creatine. The aim of this study was to evaluate the anti-inflammatory effects of creatine supplements in canine chondrocytes (CnC). CnC were stimulated with IL-1β. Release of PGE2 and TNFa was measured using ELISA. Changes in oxylipin profile was assessed using HPLC/MS. Expression of COX-2 and phosphorylated NF-kB was performed using western blot. Changes in above inflammatory responses were examined following treatment with various creatine compounds including the metabolite creatinine. COX inhibitor, Rimadyl, substantially reduced PGE2 release, despite increasing both TNFa release and COX-2 expression. All creatine compounds, including creatinine, reduced PGE2, COX-2 and TNFa in stimulated CnC. In addition, all the compounds examined reduced phosphorylated NF-kB expression. The creatine compounds were also able to interfere with the production of several oxylipins in response to IL-1β. Creatine supplements may have a beneficial role in preventing inflammation within the joint and other tissues. en_US
dc.subject Creatine, Canine, Chondrocytes, Joint en_US
dc.title Evaluation of the Anti-Inflammatory Effects of Creatine in Canine Chondrocytes as an in-vitro Model of Joint Inflammation en_US
dc.degree.discipline Pharmacology and Therapeutics en_US
dc.contributor.examiningcommittee Sitar, Daniel (Pharmacology and Therapeutics) Burczynski, Frank (Pharmacology) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note May 2016 en_US


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