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dc.contributor.supervisor Dibrov, Pavel (Microbiology) en_US
dc.contributor.author Resch, Craig
dc.date.accessioned 2016-01-18T20:12:16Z
dc.date.available 2016-01-18T20:12:16Z
dc.date.issued 2010 en_US
dc.date.issued 2011 en_US
dc.date.issued 2012 en_US
dc.date.issued 2014 en_US
dc.identifier.uri http://hdl.handle.net/1993/31098
dc.description.abstract Antiporters that exchange alkali cations (Na+ or K+) for protons play an important role in the physiology of all known bacterial species. They are involved in regulating intracellular pH and maintaining cellular volume as well as the formation of a chemical Na+ gradient across the membrane, which is important to many bacteria as an energy source for processes such as accumulation of substrates, ATP synthesis, and flagellar rotation. Another important role of cation/proton antiporters is homeostasis of intracellular cation content. The situation of a thermodynamic equilibrium of Na+ or K+ on the membrane would result in toxic intracellular levels of these cations, so bacteria have cation/proton antiporters, which expel excess of alkali cations at the expense of the proton motive force. Of many antiporters described to date, the NhaP-type family is one of the most interesting groups. Its members collectively demonstrate a great diversity of their features. There are three antiporters of NhaP type encoded in the genome of the dangerous human pathogen, Vibrio cholerae. Phenotype analysis of engineered chromosomal VcnhaP1, VcnhaP2 and VcnhaP3 deletion mutants has proven the three NhaP paralogues to be essential for V. cholerae growth at low pH in the presence of high or low concentrations of K+. Genes encoding Vc-NhaP1-3 were cloned and antiporters expressed in their functional form in an antiporter-less strains of Escherichia coli. Although initially annotated as Na+/H+ antiporters, when assayed in everted membrane vesicles, all three isoforms of Vc-NhaP were shown to be K+/H+ antiporters with only limited ability to Na+/H+ antiport. None of three proteins was able to mediate Li+/H+ exchange. Overall, the three antiporters differed in their biochemical profiles, predicted topology, and their phenotypic manifestations. en_US
dc.publisher Elsevier en_US
dc.publisher Springer en_US
dc.publisher NCR Research Press en_US
dc.publisher AMC en_US
dc.rights info:eu-repo/semantics/openAccess
dc.subject Antiporters en_US
dc.subject Microbiology en_US
dc.title Biochemistry and physiology of NhaP-type antiporters in Vibrio cholerae en_US
dc.type info:eu-repo/semantics/doctoralThesis
dc.type doctoral thesis en_US
dc.degree.discipline Microbiology en_US
dc.contributor.examiningcommittee Court, Deb (Microbiology) O'Neil, Joe (Chemistry) en_US
dc.degree.level Doctor of Philosophy (Ph.D.) en_US
dc.description.note February 2016 en_US


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