dc.contributor.supervisor |
Keijzer, Richard (Surgery) |
en_US |
dc.contributor.author |
Visser, Robin
|
|
dc.date.accessioned |
2016-01-14T15:01:33Z |
|
dc.date.available |
2016-01-14T15:01:33Z |
|
dc.date.issued |
2015 |
|
dc.identifier.uri |
http://hdl.handle.net/1993/31060 |
|
dc.description.abstract |
INTRODUCTION: Pulmonary hypoplasia causes high morbidity and mortality in congenital diaphragmatic hernia (CDH) patients. MiR-10a and miR-200b are overexpressed in human CDH lungs. We aimed to define their roles in lung development. METHODS: We profiled miR-10a expression with RT-qPCR and in situ hybridization using a nitrofen rat model for CDH. The effects of miR-10a on airway branching were evaluated in lung explants. MiR-200b’s role in airway branching was assessed in miR-200b knockout lung explants. Crossing miR-200b knockout mice with CFP-E-Cadherin was used to evaluate miR-200b’s effects on epithelial differentiation. RESULTS: Expression of miR-10a was altered in the nitrofen model and miR-10a mimics reversed lung hypoplasia in vitro. Heterozygous miR-200b lung explants displayed reduced airway branching. CFP-E-Cadherin/miR-200b knockout lung explants showed reduced epithelial expression. CONCLUSION: Both miR-10a and miR-200b are critical for lung development and CDH. Normalizing their expression may reverse lung hypoplasia and reduce the associated morbidity and mortality in CDH. |
en_US |
dc.rights |
info:eu-repo/semantics/openAccess |
|
dc.subject |
congenital diaphragmatic hernia |
en_US |
dc.subject |
lung development |
en_US |
dc.subject |
miR-10a |
en_US |
dc.subject |
miR-200b |
en_US |
dc.subject |
pulmonary hypoplasia |
en_US |
dc.title |
MiRacles for babies with pulmonary hypoplasia: the effects of miR-10a and miR-200b on lung development |
en_US |
dc.type |
info:eu-repo/semantics/masterThesis |
|
dc.degree.discipline |
Surgery |
en_US |
dc.contributor.examiningcommittee |
Boyd, April (Surgery) Pathak, Alok (Surgery) Ghavami, Saied (Human Anatomy and Cell Science) |
en_US |
dc.degree.level |
Master of Science (M.Sc.) |
en_US |
dc.description.note |
February 2016 |
en_US |