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dc.contributor.supervisorLeygue, Etienne (Biochemistry and Medical Genetics)en
dc.contributor.authorGuo, Jimin
dc.date.accessioned2008-09-26T19:45:05Z
dc.date.available2008-09-26T19:45:05Z
dc.date.issued2008-09-26T19:45:05Z
dc.identifier.urihttp://hdl.handle.net/1993/3097
dc.description.abstractThe steroid receptor RNA activator gene (SRA1) generates two distinct entities. SRA RNA coactivates several NRs whereas SRA protein (SRAP) is suspected to regulate the activity of several transcription factors, including estrogen receptors (ER). Splicing of SRA intron-1 is the major event defining SRAP coding frame. Fully spliced, coding SRA and intron-1 retained, non-coding SRA coexist in breast cancer cells. The relative proportion between the two types of SRA RNA maintains a balance between two genetically linked entities, SRA and SRAP. In this study, a minigene model was used to demonstrate that the primary sequence of SRA exon-1-intron-1-exon-2 is sufficient for alternative splicing of SRA intron-1. In addition, a modified oligoribonucleotidic construct promotes SRA intron-1 retention in breast cancer cells. This oligoribonucleotide differentially alters estradiol-induced transcription of ER regulated genes. Together, results presented herein demonstrate that the SRA-SRAP balance, which can be artificially modified by targeting alternative splicing of SRA intron-1, might be a new critical target to treat breast cancer patients.en
dc.format.extent19529391 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectsteroid receptor RNA activatoren
dc.subjectestrogen receptoren
dc.subjectalternative splicingen
dc.subjectbreast canceren
dc.titleThe effect of SRA intron-1 splicing on differential ratio of SRA-SRAP levels and on ER-mediated transcription in breast cancer cellsen
dc.typeinfo:eu-repo/semantics/masterThesis
dc.typemaster thesisen_US
dc.degree.disciplineBiochemistry and Medical Geneticsen_US
dc.contributor.examiningcommitteeAmara, Francis (Biochemistry and Medical Genetics) Xie, Jiuyong (Physiology)en
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.noteFebruary 2009en


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