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Generation of a human Middle East respiratory syndrome coronavirus (HCoV-MERS) infectious clone system by recombination of bacterial artificial chromosomes

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dc.contributor.supervisor Court, Deborah (Microbiology) Theriault, Steven (Microbiology) en_US
dc.contributor.author Nikiforuk, Aidan
dc.date.accessioned 2015-07-28T14:56:19Z
dc.date.available 2015-07-28T14:56:19Z
dc.date.issued 2015
dc.identifier.uri http://hdl.handle.net/1993/30635
dc.description.abstract Coronaviruses have caused high pathogenic epidemics within the human population on two occasions; in 2003 a coronavirus (HCoV-SARS) caused severe acute respiratory syndrome and in 2012 a novel coronavirus emerged named Middle East respiratory syndrome (HCoV-MERS). Four other species of coronavirus circulate endemically in the human population (HCoV-229E, HCoV-OC43, HCoV-NL63 and HCoV-HKU1), which cause more benign respiratory disease than either HCoV-SARS or HCoV-MERS. The emergence of HCoV-MERS provides an additional opportunity to study the characteristics of coronaviruses. Reverse genetics can be used to study an organism’s phenotype by logical mutation of its genotype. Construction of an infectious clone construct provides a means to investigate the nature of HCoV-MERS by reverse genetics. An HCoV-MERS infectious cDNA clone system was constructed to use for reverse genetics by homologous recombination of bacterial artificial chromosomes (BACs). This system should aid in answering remaining questions of coronavirus genetics and evolution as well as expedite the development of vaccines and prophylactic treatments for HCoV-MERS. en_US
dc.subject Middle East respiratory syndrome en_US
dc.subject Reverse genetics en_US
dc.subject Coronavirus en_US
dc.subject Homologous recombination en_US
dc.title Generation of a human Middle East respiratory syndrome coronavirus (HCoV-MERS) infectious clone system by recombination of bacterial artificial chromosomes en_US
dc.degree.discipline Microbiology en_US
dc.contributor.examiningcommittee Brassinga, Karen (Microbiology) Kobasa, Darwyn (Medical Microbiology) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note October 2015 en_US


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