Dietary means for enhanced gastrointestinal health and function in weaned pigs: An evaluation of carbohydrase enzymes targeting non-starch polysaccharides

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dc.contributor.supervisor Nyachoti, Martin (Animal Science) en
dc.contributor.author Kiarie, Elijah
dc.date.accessioned 2008-05-07T19:02:43Z
dc.date.available 2008-05-07T19:02:43Z
dc.date.issued 2008-05-07T19:02:43Z
dc.identifier.citation E. Kiarie, B. A. Slominski, D. O. Krause, and C. M. Nyachoti. 2008. Non-starch polysaccharide hydrolysis products of soybean and canola meal protect against enterotoxigenic Escherichia coli in piglets. J. Nutr. 138: 502-508. en
dc.identifier.citation E. Kiarie, D. O Krause, and C. M. Nyachoti. Net fluid and electrolyte losses in enterotoxigenic Escherichia coli-infected piglet small intestine upon perfusion with fumaric acid, zinc oxide, egg yolk antibodies or carbadox. Can. J. Anim. Sci. (In press). en
dc.identifier.citation E. Kiarie, C. M. Nyachoti, B. A. Slominski, and G. Blank. 2007. Growth performance, gastrointestinal microbial activity and nutrient digestibility in early-weaned pigs fed diets containing flaxseed and carbohydrase enzyme. J. Anim. Sci. 85: 2982-2993. en
dc.identifier.uri http://hdl.handle.net/1993/3038
dc.description.abstract A major challenge for the pig industry is to formulate starter diets that primarily fit the digestive capacity, maintain GIT health and promote growth without recourse to in-feed antimicrobials. Experiments were conducted to evaluate the efficacy of carbohydrase enzymes (CE) targeting non-starch polysaccharides (NSP) in enhancing gut health and function in piglets. First, an experiment was conducted to evaluate the effects of adding CE in piglet diets on growth performance, GIT bacterial activity and nutrient digestibility. Pigs fed diets containing CE had a higher ileal lactobacilli count, total organic acids concentrations, NSP digestibility and low ammonia compared with control. The effectiveness of CE targeting NSP was further evaluated using enterotoxigenic E. coli (ETEC) in a challenge model to evaluate the impact on gut health and function. Two approaches for the ETEC challenge were adopted; an in situ small intestine segments perfusion model and an in vivo model. Initially, a pilot study was conducted to establish and validate the in situ model. In the pilot study, conventional anti-diarrhea agents; fumaric acid, ZnO, egg yolk antibodies against ETEC K88 fimbriae and carbadox, attenuated fluid losses in ETEC-infected jejunal segments. Following the establishment of the in situ model, four experiments were conducted to study the effects NSP hydrolysis products (HP) from various feedstuffs (i.e. wheat, soybean meal, canola meal and flaxseed) on ETEC-induced secretory diarrhea. The results demonstrated that HP protected against ETEC-induced fluid and electrolyte losses. A further study was conducted to investigate the response of piglets fed diets containing HP and EYA singly or in combination upon oral challenge with ETEC. Feeding HP and EYA alone or in combination attenuated ETEC-enteritis symptoms such that piglets fed additives showed less pronounced acute phase responses and superior performance. Piglets fed diets containing additives had lower gastric pH, fewer ETEC adhered to ileal mucosa and lower incidence of diarrhea. Overall, reduction of intestinal pathogens or toxic bacterial metabolites contributes to enhanced GIT health and function. These novel results expand the scope of enzyme technology in animal nutrition within the new paradigm of dietary approaches to gut health and function. en
dc.format.extent 2878245 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.rights info:eu-repo/semantics/openAccess
dc.subject Carbohydrases en
dc.subject ETEC en
dc.subject Gastrointestinal health and function en
dc.subject Non-starch polysaccharides hydrolysis products en
dc.subject Nutrient digestibility en
dc.subject Post weaning collibacillosis en
dc.subject Post weaning diarrhoea en
dc.subject Weaned pigs en
dc.title Dietary means for enhanced gastrointestinal health and function in weaned pigs: An evaluation of carbohydrase enzymes targeting non-starch polysaccharides en
dc.type info:eu-repo/semantics/doctoralThesis
dc.type doctoral thesis en_US
dc.degree.discipline Animal Science en
dc.contributor.examiningcommittee Slominski, Bogdan (Animal Science) House, James (Animal Science) Blank, Gregory (Food Science) Verstegen, Martin (Wageningen University) en
dc.degree.level Doctor of Philosophy (Ph.D.) en
dc.description.note May 2008 en

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