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dc.contributor.authorAdewoye, LO
dc.contributor.authorWorobec, EA
dc.date.accessioned2007-10-10T18:11:18Z
dc.date.available2007-10-10T18:11:18Z
dc.date.issued1999-12-31
dc.identifier.citation0008-4166; CAN J MICROBIOL, DEC 1999, vol. 45, no. 12, p.1033 to 1042.en
dc.identifier.urihttp://hdl.handle.net/1993/2928
dc.description.abstractIn response to low extracellular glucose concentration, Pseudomonas aeruginosa induces the expression of the outer membrane carbohydrate-selective OprB porin. The promoter region of the oprB gene was cloned into a lacZ transcriptional fusion vector, and the construct was mobilized into P. aeruginosa OprB-deficient strain, WW100, to evaluate additional environmental factors that influence OprB porin gene expression. Growth temperature, pH of the growth medium, salicylate concentration, and carbohydrate source were found to differentially influence porin expression. This expression pattern was compared to those of whole-cell [C-14]glucose uptake under conditions of high osmolarity, ionicity, variable pH, growth temperatures, and carbohydrate source. These studies revealed that the high-affinity glucose transport genes are down-regulated by salicylic acid, differentially regulated by pH and temperature, and are specifically responsive to exogenous glucose induction.en
dc.format.extent1024656 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoengen_US
dc.rightsNo part of the NRC Research Press electronic journals may be reproduced, stored, or transmitted in any form or by any means, without the written permission of the publisher, except as stated below. Under the Canadian Copyright Act, individuals may download or print single copies of articles for personal research or study. Any person may reproduce short excerpts from articles in the journals for any purpose that respects the moral rights of authors, provided that the source is fully acknowledged. As a courtesy, the consent of authors of such material should be obtained directly from the author. Authorization to reproduce items for other than personal research or study, as stated above, may be obtained via Access © upon payment of the copyright fee of $10.00 per copy. NRC Research Press also extends certain additional rights to authors. The above rights do not extend to copying or reproduction for general distribution, for advertising or promotional purposes, for creating new collective works, or for resale. For such copying or reproduction, arrangements must be made with NRC Research Press.en
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPseudomonas aeruginosaen
dc.subjectOprB porinen
dc.subjectglucose transporten
dc.subjectregulationen
dc.subjectESCHERICHIA-COLI K-12en
dc.subjectOUTER-MEMBRANE PROTEINen
dc.subjectGENE-EXPRESSIONen
dc.subjectTRANSPORT-SYSTEMen
dc.subjectCHANNEL SIZEen
dc.subjectSIGNAL TRANSDUCTIONen
dc.subjectOSMOTIC REGULATIONen
dc.subjectMOLECULAR ANALYSISen
dc.subjectCYSTIC-FIBROSISen
dc.subjectPHen
dc.titleMultiple environmental factors regulate the expression of the carbohydrate-selective OprB porin of Pseudomonas aeruginosaen
dc.typejournal articleen_US
dc.typeinfo:eu-repo/semantics/article
dc.statusPeer revieweden
dc.identifier.doihttp://dx.doi.org/10.1139/w99-110


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