Show simple item record Hausner, G Inglis, G Yanke, LJ Kawchuk, LM McAllister, TA 2007-09-07T18:16:36Z 2007-09-07T18:16:36Z 2000-07-31T18:16:36Z
dc.identifier.citation 0008-4026; CAN J BOT, JUL 2000, vol. 78, no. 7, p.917 to 927. en
dc.description.abstract Anaerobic chytrid strains (representing four genera and seven species based on morphological characters), obtained from a variety of hosts, were analysed for variability within the 5' terminal segment of the ribosomal large subunit (LSU) gene, first internal transcribed spacer (ITS1), and the intergenic spacers (IGS) of the ribosomal repeat unit. In the analyses of the ribosomal LSU gene, strains of Neocallimastix, Orpinomyces, and Anaeromyces clustered into distinct groups, but Piromyces strains did not. Although the size of polymerase chain reaction products for the ITS1 region varied among strains, restriction fragment length polymorphism analysis was not useful in distinguishing between the strains. In the analyses of restriction fragment profiles of the IGS, strains of Orpinomyces and Anaeromyces clustered into distinct groupings that corresponded with morphological characters. In contrast, Neocallimastix strains clustered into two distinct groups. Considerable variability was observed in the IGS for strains of Piromyces. Analysis of this region split strains of Piromyces rhinzinflata obtained from a single bison into at least two distinct genetic groupings. Furthermore, the Piromyces strains obtained from bovine, wapiti, and moose clustered according to host. Overall, our molecular data suggest that morphological characters as observed under the light microscope are insufficient for the designation of phylogenetic species of Neocallimastix and Piromyces, and that integration of molecular and morphological data will be required to establish a natural classification system for the anaerobic members of the Chytridiomycota. en
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dc.language.iso en_US
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dc.subject anaerobic fungi en
dc.subject rumen en
dc.subject Chytridiomycota en
dc.subject ribosomal DNA en
dc.subject RFLP en
dc.subject RUMEN FUNGI en
dc.subject NOV-SP en
dc.subject PIROMYCES-MAE en
dc.subject GUT FUNGI en
dc.subject NEOCALLIMASTIX en
dc.subject HETEROGENEITY en
dc.subject DEGRADATION en
dc.subject MORPHOLOGY en
dc.subject GLOMALES en
dc.subject BIOLOGY en
dc.title Analysis of restriction fragment length polymorphisms in the ribosomal DNA of a selection of anaerobic chytrids en
dc.status Peer reviewed en

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