Show simple item record

dc.contributor.author Hausner, G
dc.contributor.author Inglis, G
dc.contributor.author Yanke, LJ
dc.contributor.author Kawchuk, LM
dc.contributor.author McAllister, TA
dc.date.accessioned 2007-09-07T18:16:36Z
dc.date.available 2007-09-07T18:16:36Z
dc.date.issued 2000-07-31T18:16:36Z
dc.identifier.citation 0008-4026; CAN J BOT, JUL 2000, vol. 78, no. 7, p.917 to 927. en
dc.identifier.uri http://hdl.handle.net/1993/2782
dc.description.abstract Anaerobic chytrid strains (representing four genera and seven species based on morphological characters), obtained from a variety of hosts, were analysed for variability within the 5' terminal segment of the ribosomal large subunit (LSU) gene, first internal transcribed spacer (ITS1), and the intergenic spacers (IGS) of the ribosomal repeat unit. In the analyses of the ribosomal LSU gene, strains of Neocallimastix, Orpinomyces, and Anaeromyces clustered into distinct groups, but Piromyces strains did not. Although the size of polymerase chain reaction products for the ITS1 region varied among strains, restriction fragment length polymorphism analysis was not useful in distinguishing between the strains. In the analyses of restriction fragment profiles of the IGS, strains of Orpinomyces and Anaeromyces clustered into distinct groupings that corresponded with morphological characters. In contrast, Neocallimastix strains clustered into two distinct groups. Considerable variability was observed in the IGS for strains of Piromyces. Analysis of this region split strains of Piromyces rhinzinflata obtained from a single bison into at least two distinct genetic groupings. Furthermore, the Piromyces strains obtained from bovine, wapiti, and moose clustered according to host. Overall, our molecular data suggest that morphological characters as observed under the light microscope are insufficient for the designation of phylogenetic species of Neocallimastix and Piromyces, and that integration of molecular and morphological data will be required to establish a natural classification system for the anaerobic members of the Chytridiomycota. en
dc.format.extent 548730 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.rights No part of the NRC Research Press electronic journals may be reproduced, stored, or transmitted in any form or by any means, without the written permission of the publisher, except as stated below. Under the Canadian Copyright Act, individuals may download or print single copies of articles for personal research or study. Any person may reproduce short excerpts from articles in the journals for any purpose that respects the moral rights of authors, provided that the source is fully acknowledged. As a courtesy, the consent of authors of such material should be obtained directly from the author. Authorization to reproduce items for other than personal research or study, as stated above, may be obtained via Access © upon payment of the copyright fee of $10.00 per copy. NRC Research Press also extends certain additional rights to authors. The above rights do not extend to copying or reproduction for general distribution, for advertising or promotional purposes, for creating new collective works, or for resale. For such copying or reproduction, arrangements must be made with NRC Research Press. en
dc.subject anaerobic fungi en
dc.subject rumen en
dc.subject Chytridiomycota en
dc.subject ribosomal DNA en
dc.subject RFLP en
dc.subject RUMEN FUNGI en
dc.subject NOV-SP en
dc.subject PIROMYCES-MAE en
dc.subject GUT FUNGI en
dc.subject NEOCALLIMASTIX en
dc.subject HETEROGENEITY en
dc.subject DEGRADATION en
dc.subject MORPHOLOGY en
dc.subject GLOMALES en
dc.subject BIOLOGY en
dc.title Analysis of restriction fragment length polymorphisms in the ribosomal DNA of a selection of anaerobic chytrids en
dc.status Peer reviewed en
dc.identifier.doi http://dx.doi.org/10.1139/b00-067


Files in this item

This item appears in the following Collection(s)

Show simple item record

View Statistics