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Impact of asthma, environmental exposures and ethnicity on functional responsiveness to Toll-like receptor (TLR) stimulation in children

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dc.contributor.supervisor HayGlass, Kent (Immunology) en
dc.contributor.author Lissitsyn, Yuriy V
dc.date.accessioned 2007-08-31T19:26:09Z
dc.date.available 2007-08-31T19:26:09Z
dc.date.issued 2007-08-31T19:26:09Z
dc.identifier.uri http://hdl.handle.net/1993/2765
dc.description.abstract TLRs play a key role in initiating innate immunity and in regulating the nature of the adaptive immune response. We hypothesized that functional responsiveness to TLR stimulation differs in clinically; environmentally; ethnically distinct pediatric populations. PBMC obtained from 272 children were stimulated with a panel of TLR ligands. Levels of pro- and anti-inflammatory, Th1-, Th2-associated cytokines were quantified by ELISA. We demonstrate that use of threshold concentrations of TLR4 and TLR2 ligands reveal striking differences in cytokine responses between asthmatic and non-atopic children. Specifically, non-atopic controls produce higher levels of pro-inflammatory cytokines, whereas asthmatics exhibit increased anti-inflammatory IL-10 responses. Asthmatic children exposed to environmental tobacco smoke (ETS) demonstrated elevated levels of chemokines relative to non-ETS exposed asthmatics and controls. First Nation children favor anti-inflammatory IL-10 responses, whereas Caucasian population respond to TLR activation by production of more robust pro-inflammatory and Th1 biased cytokine and chemokine responses. en
dc.format.extent 1426446 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.subject asthma en
dc.subject toll-like receptor en
dc.title Impact of asthma, environmental exposures and ethnicity on functional responsiveness to Toll-like receptor (TLR) stimulation in children en
dc.degree.discipline Immunology en
dc.contributor.examiningcommittee Becker, Allan (Pediatrics and Child Health) Marshall, Aaron (Immunology)Yang, Xi (Medical Microbiology) en
dc.degree.level Master of Science (M.Sc.) en
dc.description.note October 2007 en


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