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dc.contributor.author Rastogi, Ruchi en_US
dc.date.accessioned 2007-07-12T19:41:42Z
dc.date.available 2007-07-12T19:41:42Z
dc.date.issued 2001-08-22T00:00:00Z en_US
dc.identifier.uri http://hdl.handle.net/1993/2672
dc.description.abstract To further define the relationship between T cell activation and the nuclear import of HIV pre-integration complex (PIC), this thesis examines the role of topoisomerase II (topoII), and enzyme induced and involved in a major remodeling of nucleus during T cell activation. The negatively selected highly purified resting CD4+ human T cells, free of monocytes, were infected with HIVIIIB and stimulated with a combination of anti-CD3 and anti-CD28 monoclonal antibodies (mAbs) in the presence or absence of different topoII inhibitors (VM-26, mAMSA, novobiocin, ICRF 187) that act through different mechanisms and block the activities of both topoII_ and topoIIB isozymes. Specific mRNA expression was monitored by RT-PCR, IL-2 production by ELISA and the expression of IL-2R_ by flow cytometry. Fully reverse-transcribed viral genome was monitored by PCR amplification and the nuclear migration of PIC was monitored by semi-quantitative PCR assays of 2-LTR circles. The entry of cells into S-phase was monitored by 3H-thymidine incorporation. (Abstract shortened by UMI.) en_US
dc.format.extent 3916961 bytes
dc.format.extent 184 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.language en en_US
dc.language.iso en_US
dc.rights info:eu-repo/semantics/openAccess
dc.title Cellular control of nuclear import of HIV en_US
dc.type info:eu-repo/semantics/masterThesis
dc.type master thesis en_US
dc.degree.discipline Medical Microbiology en_US
dc.degree.level Master of Science (M.Sc.) en_US


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