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Evidence for a luminal, calcium-mediated cross talk between SERCA1 pumps and RyR1 calcium release channels in heavy sarcoplasmic reticulum, HSR, membranes

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dc.contributor.author Palahniuk, Chris G. en_US
dc.date.accessioned 2007-07-12T17:46:13Z
dc.date.available 2007-07-12T17:46:13Z
dc.date.issued 2000-06-01T00:00:00Z en_US
dc.identifier.uri http://hdl.handle.net/1993/2476
dc.description.abstract The potential for luminal Ca2+ to modulate the activity of both SERCA1 pumps and RyR1 channels was examined in isolated skeletal muscle heavy sarcoplasmic reticulum vesicles. A synchronous assay was developed using Calcium Green-2 to monitor extravesicular Ca2+ and NADH oxidation as an indicator of SERCA1 activity. Results from these assays demonstrated that SERCA1 pumps responded with near-maximal activity to RyR1-mediated Ca 2+ leak states. Therefore the activities of both the SERCA1 and the RyR1 appear intertwined through a luminal, Ca2+ mediated, cross talk, such that the formation of SERCA1-mediated luminal Ca2+ gradients required for RyR1-mediated Ca2+ releases is dependent upon the distribution of Ca2+ between free and bound luminal Ca2+ compartments. (Abstract shortened by UMI.) en_US
dc.format.extent 9215980 bytes
dc.format.extent 184 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.language en en_US
dc.language.iso en_US
dc.title Evidence for a luminal, calcium-mediated cross talk between SERCA1 pumps and RyR1 calcium release channels in heavy sarcoplasmic reticulum, HSR, membranes en_US
dc.degree.discipline Oral Biology en_US
dc.degree.level Master of Science (M.Sc.) en_US


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