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dc.contributor.author Clark, Clifford G
dc.contributor.author Chong, Patrick M
dc.contributor.author McCorrister, Stuart J
dc.contributor.author Simon, Philippe
dc.contributor.author Walker, Matthew
dc.contributor.author Lee, David M
dc.contributor.author Nguy, Kimberly
dc.contributor.author Cheng, Keding
dc.contributor.author Gilmour, Matthew W
dc.contributor.author Westmacott, Garrett R
dc.date.accessioned 2014-06-08T07:53:03Z
dc.date.available 2014-06-08T07:53:03Z
dc.date.issued 2014-03-19
dc.identifier.citation BMC Microbiology. 2014 Mar 19;14(1):70
dc.identifier.uri http://hdl.handle.net/1993/23617
dc.description.abstract Abstract Background The presence of Campylobacter jejuni temperate bacteriophages has increasingly been associated with specific biological effects. It has recently been demonstrated that the presence of the prophage CJIE1 is associated with increased adherence and invasion of C. jejuni isolates in cell culture assays. Results Quantitative comparative proteomics experiments were undertaken using three closely related isolates with CJIE1 and one isolate without CJIE1 to determine whether there was a corresponding difference in protein expression levels. Initial experiments indicated that about 2% of the total proteins characterized were expressed at different levels in isolates with or without the prophage. Some of these proteins regulated by the presence of CJIE1 were associated with virulence or regulatory functions. Additional experiments were conducted using C. jejuni isolates with and without CJIE1 grown on four different media: Mueller Hinton (MH) media containing blood; MH media containing 0.1% sodium deoxycholate, which is thought to result in increased expression of virulence proteins; MH media containing 2.5% Oxgall; and MHwithout additives. These experiments provided further evidence that CJIE1 affected protein expression, including virulence-associated proteins. They also demonstrated a general bile response involving a majority of the proteome and clearly showed the induction of almost all proteins known to be involved with iron acquisition. The data have been deposited to the ProteomeXchange with identifiers PXD000798, PXD000799, PXD000800, and PXD000801. Conclusion The presence of the CJIE1 prophage was associated with differences in protein expression levels under different conditions. Further work is required to determine what genes are involved in causing this phenomenon.
dc.title The CJIE1 prophage of Campylobacter jejuni affects protein expression in growth media with and without bile salts
dc.type Journal Article
dc.language.rfc3066 en
dc.description.version Peer Reviewed
dc.rights.holder Clifford G Clark et al.; licensee BioMed Central Ltd.
dc.date.updated 2014-06-08T07:53:04Z
dc.identifier.doi http://dx.doi.org/10.1186/1471-2180-14-70


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