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Mitochondrial BNIP3 upregulation precedes endonuclease G translocation in hippocampal neuronal death following oxygen-glucose deprivation

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dc.contributor.author Zhao, Shen-Ting
dc.contributor.author Chen, Ming
dc.contributor.author Li, Shu-Ji
dc.contributor.author Zhang, Ming-Hai
dc.contributor.author Li, Bo-Xing
dc.contributor.author Das, Manas
dc.contributor.author Bean, Jonathan C
dc.contributor.author Kong, Ji-Ming
dc.contributor.author Zhu, Xin-Hong
dc.contributor.author Gao, Tian-Ming
dc.date.accessioned 2013-10-01T19:13:12Z
dc.date.available 2013-10-01T19:13:12Z
dc.date.issued 2009-09-08
dc.identifier.citation BMC Neuroscience. 2009 Sep 08;10(1):113
dc.identifier.uri http://hdl.handle.net/1993/22214
dc.description.abstract Abstract Background Caspase-independent apoptotic pathways are suggested as a mechanism for the delayed neuronal death following ischemic insult. However, the underlying signalling mechanisms are largely unknown. Recent studies imply the involvement of several mitochondrial proteins, including endonuclease G (EndoG) and Bcl-2/adenovirus E1B 19 kDa-interacting protein (BNIP3), in the pathway of non-neuronal cells. Results In this report, using western blot analysis and immunocytochemistry, we found that EndoG upregulates and translocates from mitochondria to nucleus in a time-dependent manner in cultured hippocampal neurons following oxygen-glucose deprivation (OGD). Moreover, the translocation of EndoG occurs hours before the observable nuclear pyknosis. Importantly, the mitochondrial upregulation of BNIP3 precedes the translocation of EndoG. Forced expression of BNIP3 increases the nuclear translocation of EndoG and neuronal death while knockdown of BNIP3 decreases the OGD-induced nuclear translocation of EndoG and neuronal death. Conclusion These results suggest that BNIP3 and EndoG play important roles in hippocampal neuronal apoptosis following ischemia, and mitochondrial BNIP3 is a signal protein upstream of EndoG that can induce neuronal death.
dc.title Mitochondrial BNIP3 upregulation precedes endonuclease G translocation in hippocampal neuronal death following oxygen-glucose deprivation
dc.type Journal Article
dc.language.rfc3066 en
dc.description.version Peer Reviewed
dc.rights.holder Shen-Ting Zhao et al.; licensee BioMed Central Ltd.
dc.date.updated 2013-10-01T19:13:13Z
dc.identifier.doi http://dx.doi.org/10.1186/1471-2202-10-113


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