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Inactivation of Mycobacterium avium subspecies paratuberculosis and profiles of microbial communities during composting of livestock mortalities

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dc.contributor.supervisor Ominski, Kim (Animal Science) McAllister, Tim (Animal Science) en_US
dc.contributor.author Tkachuk, Victoria L.
dc.date.accessioned 2013-09-12T16:01:57Z
dc.date.available 2013-09-12T16:01:57Z
dc.date.issued 2013-03 en_US
dc.identifier.citation Tkachuk, V. L., Krause, D. O., McAllister, T. A., Buckley, K. E., Reuter, T., Hendrick, S. and Ominski, K. H. 2013. Assessing the inactivation of Mycobacterium avium subspecies paratuberculosis during composting of livestock carcasses. Appl. Environ. Microbiol. 79:3215-3224. en_US
dc.identifier.uri http://hdl.handle.net/1993/22186
dc.description.abstract This study explored the use of a biosecure, static composting structure to inactivate MAP. In Experiment #1, it was concluded that composting is unlikely to achieve temperatures necessary to inactivate MAP associated with cattle mortalities and that M. smegamatis is an unlikely surrogate for MAP. This study also used the same system to explore changes in the microbial community in mortality compost after exposure to thermophilic temperatures. As high-throughput sequencing technologies advance, it is possible to characterize microbial communities in environments with a high degree of resolution. In Experiment #2, as members of Clostridia were present at temperatures > 55°C, it appears that anaerobic conditions existed within regions of the compost. Extreme temperatures and non-homogeneous high moisture conditions resulted in spatial distribution of temperature in a biosecure, static composting system, which failed to meet conditions necessary for complete composting and pathogen reduction. en_US
dc.publisher Applied and Environmental Microbiology en_US
dc.subject paratuberculosis en_US
dc.subject compost en_US
dc.subject mortalities en_US
dc.subject Johne's en_US
dc.subject microbial ecology en_US
dc.subject thermophilic en_US
dc.title Inactivation of Mycobacterium avium subspecies paratuberculosis and profiles of microbial communities during composting of livestock mortalities en_US
dc.degree.discipline Animal Science en_US
dc.contributor.examiningcommittee Buckley, Katherine (Animal Science) Holley, Rick (Food Science) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note October 2013 en_US


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