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dc.contributor.supervisor Stasolla, Claudio (Plant Science) en_US
dc.contributor.author Chan, Aaron
dc.date.accessioned 2013-05-14T15:14:06Z
dc.date.available 2013-05-14T15:14:06Z
dc.date.issued 2013-05-14
dc.identifier.uri http://hdl.handle.net/1993/20393
dc.description.abstract The ability to form embryos from somatic cells or somatic embryogenesis (SE) is a form of totipotency present in plants. The effect of light on SE is poorly understood. Somatic embryogenesis of Arabidopsis thaliana in the visible region was mainly induced by red light (660 nm). The red light receptor PHYTOCHROME E (PHYE) was the only PHY mutant that reduced SE. Transcriptional analysis of phyE and darkness showed several hundred fold reduction in CONSTITUTIVE PHOTOMORPHIC DWARF (CPD) expression, a rate limiting enzyme in brassinosteroid (BR) biosynthesis. Application of BR partially rescued AGAMOUS LIKE 15 (AGL15) expression, a key SE gene, under darkness and in the phyE mutant. The results support a model where red light acts through PHYE and subsequently brassinosteroid signalling to induce SE through AGL15. en_US
dc.subject Embryogenesis en_US
dc.subject Germination en_US
dc.title Light induction of somatic embryogenesis and dark germination of Arabidopsis thaliana (L.) Heynh en_US
dc.degree.discipline Plant Science en_US
dc.contributor.examiningcommittee Ayele, Belay (Plant Science) Belmonte, Mark (Biological Sciences) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note October 2013 en_US


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