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dc.contributor.supervisorDocker, Margaret (Biological Sciences) Whyard, Steve (Biological Sciences)en_US
dc.contributor.authorHeath, George
dc.date.accessioned2013-02-12T18:22:37Z
dc.date.available2013-02-12T18:22:37Z
dc.date.issued2013-02-12
dc.identifier.urihttp://hdl.handle.net/1993/16593
dc.description.abstractThe sea lamprey (Petromyzon marinus) is an invasive pest in the Laurentian Great Lakes. Current pest control programs for sea lamprey are expensive and can produce off-target effects. RNA interference (RNAi) based technologies have the potential to augment existing sea lamprey control programs. In this study, sea lamprey embryos and larvae (10-100 mm) were treated with small interfering RNAs (siRNAs) targeting housekeeping genes (β actin, α actinin, calmodulin, elongation factor 1α, splicing factor 1 and γ tubulin) and gene expression was measured. Three of the siRNA embryo treatments (α-actinin, calmodulin, splicing factor 1) produced significant knockdown and increased mortality while treatment with tubulin siRNA produced only knockdown. Larval siRNA treatments produced knockdown of four genes (α-actinin, calmodulin, elongation factor 1α, splicing factor 1) and increased mortality with tubulin-siRNA treatments. Differential effects of siRNA treatment across life stage and gene target are discussed. These results suggest that siRNAs have potential uses as species-specific pesticides in sea lamprey.en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectlampreyen_US
dc.subjectRNAien_US
dc.titleNew opportunities in pest control: Sea lamprey and small RNA technologiesen_US
dc.typeinfo:eu-repo/semantics/masterThesis
dc.typemaster thesisen_US
dc.degree.disciplineBiological Sciencesen_US
dc.contributor.examiningcommitteeCivetta, Alberto (Biochem. and Medical Genetics) Marcus, Jeffrey (Biological Sciences)en_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.noteFebruary 2013en_US


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